Cloning and identification of a novel variant of human vascular endothelial growth factor

Abstract

A novel variant of human vascular endothelial growth factor (h’VEGF165) cDNA was amplified by nested PCR method from the HL60¹ cells and was cloned into a eukaryotic expressing vector pcDNA₃ to construct a recombinant plasmid pCD-h’VEGF165. The amplified h’VEGF165 cDNA fragment was identified by enzyme digestion and DNA sequencing methods. Also, wild-type hVEGF165 cDNA was obtained, identified and cloned into a eukaryotic expressing vector pcDNA3 by using the same methods. The results of DNA sequencing showed that h’VEGF165 cDNA cloned from HL60¹ was 600 bp in size with 8 % of the base sequence in h’VEGF165 cDNA being changed as compared with the base sequence in the wild-type hVEGF165 cDNA. The results of sequencing of hVEGF165 which was cloned from HL60 by us were consistent with the reports completely.

Keywords

human vascular endothelia growth factor / nested PCR / molecular clone

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