Spermicidal effect of jieze No. 1 in combination with nonoxynol-9In vitro

Abstract

Spermicidal effect of Jieze No. 1 (JZ1) in combination with nonoxynol-9 (N-9) was examinedin vitro. The minimum spermicidal concentration of JZ1 decoction, N-9 and their mixture solution in 20 s and 3 min were examined by improved spermicidal test of Sander-cramerin vitro. The percentages of progressively moving spermatozoa, moving spermatozoa and viable spermatozoa were also observed 20 s, 3 min and 30 min after the addition of the liquid medicine. Our results showed that sperms did not recover their activities in a revival test when the minimum spermicidal concentration of either JZ1 decoction, or N-9, or the mixed solution of the two agents, was used. N-9 (JZ1 in the mixed group) showed significant differences in the percentages of progressively moving spermatozoa, moving spermatozoa, and visible spermatozoa in 20 s, 3 min, and 30 min, when compared with N-9 alone (P<0.01). We are led to conclude that JZ1 decoction can improve N-9 spermicidal actionin vitro, and when used in combination with N-9, it has synergic effect.

Keywords

Jieze No. 1 / nonoxynol-9 / sperm / contraception / in vitro

References

[1] ErnyR, PorteH. Sexually transmitted diseases (STD) and contraception. Fertil Contracept Sex, 1989, 17(6): 503-503

[2] RoddyR E, CorderoM. A randomized controlled trial comparing nonoxynol-9 lubricated condoms with silicone lubricated condoms for prophylaxis. Sex Transm Infect, 1998, 74(2): 116-116

[3] RichardsonB A, MartinH L, StevensCE, et al. . Use of nonoxynol-9 and changes in vaginal lactobacilli. J Infect Dis, 1998, 178(2): 441-441

[4] YuY. Advantages and disadvantages of contraceptive methods to the prevention of STD. Guo Wai Yi Xue Ji Hua Sheng Yu Xue Fen Ce (Chinese), 1996, 46-6

[5] WHO. Laboratory manual for the examination of human semen and sperm cervical mucus interaction, 19923rd ed.Cambridge, Cambridge University Press

[6] SanderF V, CramerS D. A practical method for testing the spermicidal action of contraceptives. Human Fertility, 1941, 6134-134

[7] XuJ X. Barrier method, spermicide and natural contraceptive methods. J Prac Obstet Gynecol (Chinese), 2002, 18(6): 332-332