Longitudinal cell mediated immune responses and cross reactivity of lymphocytes from rabbits infected with Candida albicans

Abstract

A comparison between longitudinal cellular and humoral immune response to C. albicans antigen in immunized rabbits was performed. The results showed that the peak of lymphocyte transformation occurred on the 18th day post immunization. Low level of antibodies (IgG), as detected in the ELISA assay, was found by 28th day post immunization.

The relevance of cross-reactivity between the antigens from C. albicans and other five pathogenic Candida species was studied. Rabbits infected with C. albicans reacted in the leukocyte transformation test in varying degrees to C. albicans, as well as to the antigen extracts of other Candida species. The results obtained suggest that the six pathogenic species of Candida can be divided into three groups according to the results of in vitro test for cell mediated immune cross-reaction. The cell wall antigens from different Candida species revealed strong cross-reactions between species, while the cytoplasmic antigens appeared to be more specific.

Keywords

Candida / albicans / cellular immune response / humoral immune response / kinetics / cross-reactivity

References

[1] BiceDE, et al. . Comparison of Candidadelayed hypersensitivity skin test size with lymphocyte transformation, migration inhibitory factor production and antibody titre. Int Arch Allergy, 1974, 4754-62

[2] OddsFC. Candida and Candidosis, 1979, London, Leicester University Press: 209-27

[3] JenssenHL, et al. . Cell electrophoretic studies on the cellular immune response to C. albicans in rabbits. Sabouraudia, 1975, 13123-31

[4] JonsenJ. Serological studies in fungi: Comparison of agglutinins and precipitins in anti-candida sera. Acta Pathol Microbiol Scand, 1955, 3779-83

[5] ShiYE. Isolation and characterization of cell wall and cytoplasmic antigens and peripheral blood leukocyte responses in rabbits immunized with Candida albicans. Acta Acad Med Wuhan, 1985, 578-85

[6] VollerA, et al. . Enzyme-linked immunosorbent assay. Manual of Clinical Immunology, 1981ed. 3Washington, Am Soc Microbiol: 359-71

[7] Zawisza-ZenktelerW, Zamiechowska-MiazgaJ. Response of peripheral blood lymphocytes from rabbits to Candida albicans antigens. Bull Acad Polon Sci, 1974, 22293-8

[8] KagayaK, et al. . Murine defense mechanism against C. albicans infection. I. Collaboration of cell-mediated and humoral immunities in protection against systemic C. albicans infection. Microbiol Immunol, 1981, 25647-54

[9] HasencleverHF, et al. . Antigenic studies of Candida. I. Antigenic relation of C. albicans group A and group B to Candida stellatoidea and Candida tropicalis. J Bacteriol, 1961, 82574-7