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Frontiers of Environmental Science & Engineering

Front. Environ. Sci. Eng.    2016, Vol. 10 Issue (2) : 362-367
Approaching the binding between Cu(II) and aerobic granules by a modified titration and µ-XRF
Hongwei LUO,Longfei WANG,Zhonghua TONG,Hanqing YU,Guoping SHENG()
CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei 230026, China
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Interactions between metals and activated sludge can substantially affect the fate and transport of heavy metals in wastewater treatment plants. Therefore, it is important to develop a simple, fast and efficient method to elucidate the interaction. In this study, a modified titration method with a dynamic mode was developed to investigate the binding of Cu(II), a typical heavy metal, onto aerobic granules. The titration results indicated that pH and ionic strength both had a positive effect on the biosorption capacity of the granular sludge. The µ-XRF results demonstrated that the distribution of metals on the granular surface was heterogeneous, and Cu showed strong correlations and had the same “hot spots” positions with other metal ions (e.g., Ca, Mg, Fe etc.). Ion exchange and complexing were the main mechanisms for the biosorption of Cu(II) by aerobic granules. These results would be beneficial for better understanding of Cu(II) migration and its fate in wastewater treatment plants.

Keywords aerobic granules      Cu(II)      modified titration      µ-XRF analysis     
Corresponding Authors: Guoping SHENG   
Online First Date: 02 July 2015    Issue Date: 01 February 2016
 Cite this article:   
Hongwei LUO,Longfei WANG,Zhonghua TONG, et al. Approaching the binding between Cu(II) and aerobic granules by a modified titration and µ-XRF[J]. Front. Environ. Sci. Eng., 2016, 10(2): 362-367.
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Hongwei LUO
Longfei WANG
Zhonghua TONG
Hanqing YU
Guoping SHENG
Fig.1  Cu(II) titration onto aerobic granules at different sludge concentrations using dynamic mode. Other experimental conditions: solution pH 6.5, ionic strength 0.001 mol·L−1
experimental conditions qmax/(mg·g−1) b/(L·mg−1) R2
pH ionic strength/(mol·L−1) MLSS/(g·L−1)
3.0 0.01 4.5 72.41 0.038 0.994
5.0 83.28 0.046 0.997
7.0 98.57 0.059 0.998
6.0 0.001 4.5 54.32 0.036 0.988
0.01 90.16 0.044 0.981
0.1 121.79 0.065 0.962
6.5 0.001 1.5 94.62 0.051 0.974
4.5 91.96 0.048 0.991
Tab.1  Parameters for Cu(II) titration onto aerobic granules obtained from nonlinear regression of the titration data at the experimental conditions
Fig.2  Influences of (a) pH and (b) ionic strength on the Cu(II) titration onto aerobic granules using a dynamic mode
Fig.3  Synchrotron-based µ-XRF mapping images showing the distribution of heavy metals on the granule after Cu(II) titration
ions metal released/Cu uptake amounts/(mg·g−1) metal released in control test/(mg·g−1)
Ca 5.95 0.04
Mg 2.05 0.02
Na 0.54 0.25
K 7.18 0.40
Cu 21.87
Tab.2  Released amounts of different light metal ions during Cu(II) biosorption by aerobic granules
Fig.4  ATR-FTIR spectra of aerobic granules before and after Cu(II) biosorption
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