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Frontiers of Environmental Science & Engineering

Front Envir Sci Eng    2013, Vol. 7 Issue (6) : 844-850     https://doi.org/10.1007/s11783-013-0487-2
RESEARCH ARTICLE |
Degradation of bisphenol A by microorganisms immobilized on polyvinyl alcohol microspheres
Xue BAI1,2, Hanchang SHI1(), Zhengfang YE3, Qiujin SUN1, Qing WANG1, Zhongyou WANG3
1. State Key Joint Laboratory of Environmental Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China; 2. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lake (Ministry of Education), College of Environmental Science and Engineering, Hohai University, Nanjing 210098, China; 3. The Key Laboratory of Water and Sediment Sciences (Ministry of Education), Department of Environmental Engineering, Peking University, Beijing 100871, China
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Abstract

In this study, microorganisms (named B111) were immobilized on polyvinyl alcohol microspheres prepared by the inverse suspension crosslinked method. The biodegradation of bisphenol A (BPA) and 4-hydroxybenzaldehyde, a degradation product of BPA, by free and immobilized B111 was investigated. The BPA degradation studies were carried out at initial BPA concentrations ranging from 25 to 150 mg·L-1. The affinity constant Ks and maximum degradation rate Rmax were 98.3 mg·L-1 and 19.7 mg·mg-1VSS·d-1 for free B111, as well as 87.2 mg·L-1 and 21.1 mg·mg-1VSS·d-1 for immobilized B111, respectively. 16S rDNA gene sequence analyses confirmed that the dominant genera were Pseudomonas and Brevundimonas for BPA biodegradation in microorganisms B111.

Keywords bisphenol A      polyvinyl alcohol      immobilized microorganisms      4-hydroxybenzaldehyde     
Corresponding Authors: SHI Hanchang,Email:hanchang@mail.tsinghua.edu.cn   
Issue Date: 01 December 2013
 Cite this article:   
Xue BAI,Hanchang SHI,Zhengfang YE, et al. Degradation of bisphenol A by microorganisms immobilized on polyvinyl alcohol microspheres[J]. Front Envir Sci Eng, 2013, 7(6): 844-850.
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http://journal.hep.com.cn/fese/EN/10.1007/s11783-013-0487-2
http://journal.hep.com.cn/fese/EN/Y2013/V7/I6/844
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Xue BAI
Hanchang SHI
Zhengfang YE
Qiujin SUN
Qing WANG
Zhongyou WANG
Fig.1  Schematic of the immobilization of microorganisms on PVA microspheres
Fig.2  Chromatograms of BPA metabolites degraded by free (a) and immobilized (b) B111
Fig.3  Average rates of BPA (a) and PHBA (b) degradation by free and immobilized B111
chemicalsmicroorganismsKs/(mg·L-1)RmaxRmax/KsR2
specific to volume/(mg·L-1·d-1)specific to microorganisms/(mg·mg-1 VSS·d-1)
BPAfree B11198.310.719.70.110.9942
immobilized B11187.211.521.10.130.9893
PHBAfree B111103.812.322.50.120.9951
immobilized B11196.515.929.20.160.9916
Tab.1  Kinetic parameters for the free and immobilized B111 degradation of BPA and PHBA
Fig.4  DGGE profiles of amplified 16S rDNA fragments from free (a) and immobilized (b) B111
Fig.5  Phylogenetic trees for the partial bacterial 16S rDNA sequences
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