Establishment of efficient Trichosanthes mottle mosaic virus-derived gene silencing in cucurbit plants

Abstract

The Cucurbitaceae family includes a wide range of economically important fruits and vegetables; however, the laborious and highly inefficient genetic transformation efficacy of cucurbits has hindered the exploration of their gene functions. Virus-induced gene silencing (VIGS) technology, employed from the antiviral RNA silencing defense, has emerged as a viable alternative for high-throughput study of plant gene function. In this study, we successfully established a VIGS system utilizing Trichosanthes mottle mosaic virus (TrMMV), a new member of the genus Tobamovirus. We demonstrated the high efficacy and durability of gene silencing mediated by the TrMMV-VIGS vector in Nicotiana benthamiana, as well as in several cucurbit species, including Cucurbita pepo, Cucumis sativus, C. lanatus, and C. melo. The insertion of 90–400 bp fragments into the vector led to effective silencing of the target gene in both C. sativus and C. melo, with a notably higher silencing efficiency observed in C. melo. Furthermore, the TrMMV-VIGS vector induced a pronounced photobleaching phenotype in the flowers of C. melo, underscoring its potential application in functional genomic research concerning floral traits in this particular species. Taken together, the TrMMV-VIGS system developed herein will facilitate rapid and high-throughput identification of gene functions in cucurbit crops.

Keywords

Trichosanthes mottle mosaic virus / Virus-induced gene silencing / Cucurbits / Flower / Biological Sciences / Genetics

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Funding

National Key Research and Development Program of China(2022YFD1401200)

National Natural Science Foundation of China(32272518)

Yunnan Zhou Xueping Expert Work Station(202205AF150047)

RIGHTS & PERMISSIONS

The Author(s)