A G-type lectin receptor-like kinase TaSRLK confers wheat resistance to stripe rust by regulating the reactive oxygen species signaling pathway

Abstract

Wheat stripe rust, caused by an obligate biotrophic pathogen Puccinia striiformis f. sp. tritici (Pst) seriously threatens wheat production. Discovering and utilizing of wheat resistance genes is the most effective and economical method to control diseases. The G-type lectin receptor-like kinase (LecRLKs) involved in biotic stress perception, while their roles in wheat resistance to Pst remain elusive. In our study, we identified 398 G-type LecRKs in wheat through BLAST and HMM profiling. The transcript level of 16 random selected G-type LecRKs from each subfamily were analyzed and found TaSRLK is highly induced by avirulent Pst CYR23 infection. TaSRLK-silenced wheat plants showed reduced resistance to Pst with increased hyphal length and decreased H₂O₂ accumulation. Surprisingly, TaSRLK was localized to the chloroplast and can induce cell death in Nicotiana benthamiana. Further, TaSRLK was shown to interact with and phosphorylate a peroxidase TaPrx1. Importantly, TaPrx1 involved in wheat resistance to Pst through regulating reactive oxygen species (ROS) production. Together these findings demonstrate that TaSRLK positively modulates ROS-associated wheat resistance by binding with TaPrx1.

Keywords

Receptor like kinases / Peroxidase / Wheat stripe rust / Reactive oxygen species / Agricultural and Veterinary Sciences / Crop and Pasture Production / Biological Sciences / Genetics

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Funding

National Natural Science Foundation of China(32202262)

Gansu Provincial Youth Science and Technology Fund(23JRRA1433)

Doctoral Scientific Research Starting Foundation of Baoji University of Arts and Science(GAU-KYQD-2022-28)

RIGHTS & PERMISSIONS

The Author(s)