Rescuing the Golgi from heat damages by ATG8: restoration rather than clean-up

Abstract

High temperature stress poses significant adverse effects on crop yield and quality. Yet the molecular mechanisms underlying heat stress tolerance in plants/crops, especially regarding the organellar remodeling and homeostasis, are largely unknown. In a recent study, Zhou et al. reported that autophagy-related 8 (ATG8), a famous regulator involved in autophagy, plays a new role in Golgi restoration upon heat stress. Golgi apparatus is vacuolated following short-term acute heat stress, and ATG8 is translocated to the dilated Golgi membrane and interacts with CLATHRIN LIGHT CHAIN 2 (CLC2) to facilitate Golgi restoration, which is dependent on the ATG conjugation system, but not of the upstream autophagic initiators. These exciting findings broaden the fundamental role of ATG8, and elucidate the organelle-level restoration mechanism of Golgi upon heat stress in plants.

Keywords

ATG8 / Autophagy / CLC2 / ER / Golgi restoration / Heat stress

References

[1] BuF, YangMK, GuoX, HuangW, ChenL. Multiple functions of ATG8 family proteins in plant autophagy. Front Cell Dev Biol, 2020, 8: 466

[2] Che P, Bussell JD, Zhou WX, Estavillo GM, Pogson BJ, Smith SM (2010) Signaling from the endoplasmic reticulum activates brassinosteroid signaling and promotes acclimation to stress in Arabidopsis. Sci Signal 3:ra69. https://doi.org/10.1126/scisignal.2001140

[3] HanSJ, YuBJ, WangY, LiuYL. Role of plant autophagy in stress response. Protein Cell, 2011, 2: 784-791

[4] JiaM, LiuX, XueH, WuY, ShiL, WangR, ChenY, XuN, ZhaoJ, ShaoJ, et al.. Noncanonical ATG8-ABS3 interaction controls senescence in plants. Nat Plant, 2019, 5: 212-224

[5] LiJY, YangC, XuJ, LuHP, LiuJX. The hot science in rice research: How rice plants cope with heat stress. Plant Cell Environ, 2023, 46: 1087-1103

[6] LiuXH, LyuYS, YangW, YangZT, LuSJ, LiuJX. A membrane-associated NAC transcription factor OsNTL3 is involved in thermotolerance in rice. Plant Biotechnol J, 2020, 18: 1317-1329

[7] SunL, LuSJ, ZhangSS, ZhouSF, SunL, LiuJX. The lumen-facing domain is important for the biological function and organelle-to-organelle movement of bZIP28 during ER stress in Arabidopsis. Mol Plant, 2013, 6: 1605-1615

[8] SunL, ZhangSS, LuSJ, LiuJX. Site-1 protease cleavage site is important for the ER stress-induced activation of membrane-associated transcription factor bZIP28 in Arabidopsis. Sci China-Life Sci, 2015, 58: 270-275

[9] SunJL, LiJY, WangMJ, SongZT, LiuJX. Protein quality control in plant organelles: Current progress and future perspectives. Mol Plant, 2021, 14: 95-114

[10] ZhangJY, LiXM, LinHX, ChongK. Crop improvement through temperature resilience. Annu Rev Plant Biol, 2019, 70: 753-780

[11] ZhangH, ZhouJF, KanY, ShanJX, YeWW, DongNQ, GuoT, XiangYH, YangYB, LiYC, et al.. A genetic module at one locus in rice protects chloroplasts to enhance thermotolerance. Science, 2022, 376: 1293-1300

[12] ZhouJ, MaJ, YangC, ZhuX, LiJ, ZhengX, LiX, ChenS, FengL, WangP, et al.. A non-canonical role of ATG8 in Golgi recovery from heat stress in plants. Nat Plant, 2023, 9: 749-765

[13] ZhuJK. Abiotic stress signaling and responses in plants. Cell, 2016, 167: 313-324

Funding

State Key Project of Research and Development Plan(Development Plan)