DNA sequencing using nanopores and kinetic proofreading

Abstract

We discuss the feasibility of using a nanopore sandwich device to implement the principle of kinetic proofreading to discriminate incorrect hybridizing oligonucleotides on a target DNA or RNA. We propose a method of sequencing DNA or RNA using this approach. The design parameters for such a DNA sequencer are estimated from the Hopfield-Ninio theory of kinetic proofreading and Schrödinger’s first-passage-time distribution function.

Graphical abstract

Keywords

DNA sequencing / nanopore sequencing / biosensing using kinetics

References

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