The measurement of circulating blood volume (CBV) is crucial in various medical conditions including surgery, iatrogenic problems, rapid fluid administration, transfusion of red blood cells, or trauma with extensive blood loss including battlefield injuries and other emergencies. Currently, available commercial techniques are invasive and time-consuming for trauma situations. Recently, we have proposed high-speed multi-wavelength photoacoustic/photothermal (PA/PT) flow cytometry for in vivo CBV assessment with multiple dyes as PA contrast agents (labels). As the first step, we have characterized the capability of this technique to monitor the clearance of three dyes (indocyanine green, methylene blue, and trypan blue) in an animal model. However, there are strong demands on improvements in PA/PT flow cytometry. As additional verification of our proof-of-concept of this technique, we performed optical photometric CBV measurements in vitro. Three label dyes—methylene blue, crystal violet and, partially, brilliant green—were selected for simultaneous photometric determination of the components of their two-dye mixtures in the circulating blood in vitro without any extra data (like hemoglobin absorption) known a priori. The tests of single dyes and their mixtures in a flow system simulating a blood transfusion system showed a negligible difference between the sensitivities of the determination of these dyes under batch and flow conditions. For individual dyes, the limits of detection of 3×10–6 M‒3×10–6 M in blood were achieved, which provided their continuous determination at a level of 10–5 M for the CBV assessment without a priori data on the matrix. The CBV assessment with errors no higher than 4% were obtained, and the possibility to apply the developed procedure for optical photometric (flow cytometry) with laser sources was shown.
In order to enhance the signal-to-noise-ratio of a distributed acoustic sensing system based on phase-sensitive optical time-domain reflectometry (Φ-OTDR), we have proposed a combination of segmented unwrapping algorithm, averaging estimation of phase difference, and infinite impulse response (IIR) filtering method. The enhancement of signal quality is numerically demonstrated. Moreover, we have studied the influence resulted from the light source noise on the Φ-OTDR performance. The result has shown that when the linewidth of light source used in the Φ-OTDR system is narrower, the performance of the system is better. In a word, such a Φ-OTDR system could obtain higher quality demodulated signals when the narrower linewidth light source is chosen and the method of averaging estimation phase difference is used.
Bridge is an important part of modern transportation systems and deformation is a key index for bridge’s safety evaluation. To achieve the long span bridge curve measurement rapidly and timely and accurately locate the bridge maximum deformation, the continuous deformation measurement system (CDMS) based on inertial platform is presented and validated in this paper. Firstly, based on various bridge deformation measurement methods, the method of deformation measurement based on the fiber optic gyro (FOG) is introduced. Secondly, the basic measurement principle based on FOG is presented and the continuous curve trajectory is derived by the formula. Then the measurement accuracy is analyzed in theory and the relevant factors are presented to ensure the measurement accuracy. Finally, the deformation measurement experiments are conducted on a bridge across the Yangtze River. Experimental results show that the presented deformation measurement method is feasible, practical, and reliable; the system can accurately and quickly locate the maximum deformation and has extensive and broad application prospects.
This paper presents a new technique for flat optical frequency comb (OFC) generation, which is based on the nonlinear process of multiple four-wave mixing (FWM) effects. The nonlinear effects are significantly enhanced by using the proposed optical feedback scheme consisting of a single mode fiber (SMF), two highly nonlinear fibers (HNLFs) with different zero dispersion wavelengths (ZDWs) and polarization beam splitters (PBSs). Simulation results illustrate its efficiency and applicability of expanding a comb to 128 coherent lines spaced by only 20 GHz within 6-dB power deviation.