RING finger protein 152 (RNF152) is a novel RING finger protein and has not been well characterized. We report here that RNF152 is a canonical RING finger protein and has E3 ligase activity. It is polyubiqitinated partly through Lys-48-linked ubiquitin chains in vivo and this phenomenon is dependent on its RING finger domain and transmembrane domain. RNF152 is localized in lysosomes and co-localized with LAMP3, a lysosome marker. Moreover, over-expression of RNF152 in Hela cells induces apoptosis. These results suggest that RNF152 is a lysosome localized E3 ligase with pro-apoptotic activities. It is the first E3 ligase identified so far that is involved in lysosome-related apoptosis.
Songling Zhang, Wei Wu, Yanfang Wu, Jiyan Zheng, Talin Suo, Hong Tang, Jie Tang,
. RNF152, a novel lysosome localized E3 ligase
with pro-apoptotic activities[J]. Protein & Cell, 2010
, 1(7)
: 656
-663
.
DOI: 10.1007/s13238-010-0083-1