Effect of male age at the time of irradiation on the sexual performance of sterile Ceratitis capitata males: insights from remating female offspring

Abstract

The Sterile Insect Technique (SIT) is an effective strategy for controlling insect pests, such as the Mediterranean fruit fly (Ceratitis capitata, Wiedemann). The effectiveness of the SIT depends on the ability of the sterile males to mate and their capacity to induce sterility in wild females. This study evaluated how the irradiation age affects their sexual performance, measured by the outcome of female remating events. Males of the GSS VIENNA 8^D53− were irradiated at eleven different ages, from 72 h before emergence (pupal stage) to 72 h after emergence (adult stage) and mated with wild females. These females were subsequently allowed to mate with fertile males from the fluorescent TSS VIENNA 8 1260. The presence of fluorescent offspring was used as indicator of second-male paternity. Results showed that males irradiated at post-emergence ages produced the lowest egg-to-pupae conversion rate, indicating a greater ability to prevent females from producing offspring after remating with a fertile male. In contrast, males irradiated at pre-emergence ages were associated with higher numbers of fluorescent offspring. Although no significant differences were found in mating competitiveness (RSI), the outcome of the remating showed differences in the effectiveness of initial matings. These findings highlight the importance of considering the age of flies at time of irradiation in mass-rearing protocols to enhance the efficacy of SIT programs targeting C. capitata and suggest that irradiating males later in their life cycle, such as adult stages or in pupae close to adult emergence, limit offspring if females remate with fertile males in the field.

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2025 The Author(s). Insect Science published by John Wiley & Sons Australia, Ltd on behalf of Institute of Zoology, Chinese Academy of Sciences.