Increased versatility and convenience: Advances and strategy optimization of Receptor-Mediated Ovary Transduction of Cargo-mediated genetic modification in insects
Xia Ling , Cao Zhou , Jun-Feng Hong , Yan-Ping Jiang , Quan Sun , Ling-Yi Li , Si-Yi Wang , Xin-Yuan Xie , Qi-Li Zou , Xiao-Lin Yang , Kai Xiang , Jin Ma , Liang Qiao , Bin Chen , Wei Sun
Insect Science ›› 2026, Vol. 33 ›› Issue (1) : 2 -12.
Genetic modification via gene editing has become a widely adopted and demonstrably effective method in functional gene research within entomology. However, the optimal efficiency and simplicity of delivering exogenous guide RNA-clustered regularly interspaced short palindromic repeats-associated protein 9 complexes into target tissues are crucial for successful gene editing. The Receptor-Mediated Ovary Transduction of Cargo (ReMOT) strategy, which simplifies the delivery process, target-site selection, technical requirements, and delivery cost compared with embryonic microinjection, enabling efficient editing at the germline level, is gaining increasing attention. Although the feasibility and advantages of this technique have been demonstrated in various insect species, further optimization of operational details and the overcoming of further bottlenecks are still required. This review focuses on advances in developing ReMOT as a valuable technology, exploring its applicability, rationale for selecting the ovary as a delivery target site, factors influencing its efficiency, and improvement recommendations. The versatility and effectiveness of ReMOT make it a promising method for researchers looking to make precise genetic modifications with greater ease and efficiency.
insect genetic modification / peptide ligand / ReMOT Control / strategy optimization
2025 Institute of Zoology, Chinese Academy of Sciences.
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