Fluorescence lifetime imaging in biosciences:
technologies and applications
NIESNER Raluca1, GERICKE Karl-Heinz2
Author information+
1.Helmholtz Centre for Infection Research; 2.Institute for Physical and Theoretical Chemistry, Technical University Braunschweig;
Show less
History+
Published
05 Mar 2008
Issue Date
05 Mar 2008
Abstract
The biosciences require the development of methods that allow a non-invasive and rapid investigation of biological systems. In this aspect, high-end imaging techniques allow intravital microscopy in real-time, providing information on a molecular basis. Far-field fluorescence imaging techniques are some of the most adequate methods for such investigations. However, there are great differences between the common fluorescence imaging techniques, i.e., wide-field, confocal one-photon and two-photon microscopy, as far as their applicability in diverse bioscientific research areas is concerned. In the first part of this work, we briefly compare these techniques. Standard methods used in the biosciences, i.e., steady-state techniques based on the analysis of the total fluorescence signal originating from the sample, can successfully be employed in the study of cell, tissue and organ morphology as well as in monitoring the macroscopic tissue function. However, they are mostly inadequate for the quantitative investigation of the cellular function at the molecular level. The intrinsic disadvantages of steady-state techniques are countered by using time-resolved techniques. Among these fluorescence lifetime imaging (FLIM) is currently the most common. Different FLIM principles as well as applications of particular relevance for the biosciences, especially for fast intravital studies are discussed in this work.
NIESNER Raluca, GERICKE Karl-Heinz.
Fluorescence lifetime imaging in biosciences:
technologies and applications. Front. Phys., 2008, 3(1): 88‒104 https://doi.org/10.1007/s11467-008-0002-6
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
This is a preview of subscription content, contact us for subscripton.
References
1. Swedlow J R Platani M Cell Struct. Funct. 2002 27335. doi: 10.1247/csf.27.335 2. Evans J A Nishioka N S Curr. Opin. Gastroenterol. 2005 21578. doi: 10.1097/01.mog.0000174217.62214.10 3. Saito F Takahama Y Tanpakushitsu KakusanKoso 2004 491592. doi: null 4. Diaspro A Chirico G Collini M Q Rev. Biophys. 2005 3897. doi: 10.1017/S0033583505004129 5. Chen Y Mills J D Periasamy A Differentiation 2003 71528. doi: 10.1111/j.1432-0436.2003.07109007.x 6. Lakowicz J R Szmacinski H Nowaczyk K Berndt K W Johnson M Analytical Biochemistry 1992 202316. doi: 10.1016/0003-2697(92)90112-K 7. Szmacinski H Nowaczyk K Berndt K Lakowicz J R FasebJournal 1992 6A35. doi: null 8. Gratton E Breusegem S Sutin J Ruan Q Barry N J Biomed. Opt. 2003 8381. doi: 10.1117/1.1586704 9. Becker W Bergmann A Hink M A Konig K Benndorf K Biskup C Microsc. Res. Tech. 2004 6358. doi: 10.1002/jemt.10421 10. Becker W Bergmann A Haustein E Petrasek Z Schwille P Biskup C Kelbauskas L Benndorf K Klocker N Anhut T Riemann I Konig K Microsc. Res. Tech. 2006 69186. doi: 10.1002/jemt.20251 11. Elson D Requejo-Isidro J Munro I Reavell F Siegel J Suhling K Tadrous P Benninger R Lanigan P McGinty J Talbot C Treanor B Webb S Sandison A Wallace A Davis D Lever J Neil M Phillips D Stamp G French P Photochem. Photobiol. Sci 2004 3795. doi: 10.1039/b316456j 12. Gerritsen H C Vroom J M de Grauw C J IEEE Eng Med. Biol. Mag. 1999 1831. doi: 10.1109/51.790989 13. Behnsen J Narang P Hasenberg M Gunzer F Bilitewski U Klippel N Rohde M Brock M Brakhage A A Gunzer M PLoS. Pathog. 2007 3e13. doi: 10.1371/journal.ppat.0030013 14. Gunzer M Riemann H Basoglu Y Hillmer A Weishaupt C Balkow S Benninghoff B Ernst B Steinert M Scholzen T Sunderkotter C Grabbe S Blood 2005 1062424. doi: 10.1182/blood-2005-01-0342 15. Gunzer M Weishaupt C Hillmer A Basoglu Y Friedl P Dittmar K E Kolanus W Varga G Grabbe S Blood 2004 1042801. doi: 10.1182/blood-2004-03-1193 16. Denk W Strickler J H Webb W W Science 1990 24873. doi: 10.1126/science.2321027 17. Inoue H Kudo S E Shiokawa A Nat. Clin. Pract. Gastroenterol. Hepatol. 2005 231. doi: 10.1038/ncpgasthep0072 18. Miyashita T Methods Mol. Biol. 2004 261399. doi: null 19. Kaufman S C Musch D C Belin M W Cohen E J Meisler D M Reinhart W J Udell I J Van Meter W S Ophthalmology 2004 111396. doi: 10.1016/j.ophtha.2003.12.002 20. Hell S W Andresen V J Microsc. 2001 202457. doi: 10.1046/j.1365-2818.2001.00918.x 21. Nielsen T Fricke M Hellweg D Andresen P J Microsc. 2001 201368. doi: 10.1046/j.1365-2818.2001.00852.x 22. Niesner R A Andresen V Neumann J Spiecker H Gunzer M Biophys. J. 2007 . doi: null 23. Egner A Hell S W Trends Cell Biol. 2005 15207. doi: 10.1016/j.tcb.2005.02.003 24. Kano H Jakobs S Nagorni M Hell S W Ultramicroscopy 2001 90207. doi: 10.1016/S0304-3991(01)00132-2 25. Hell S W Schrader M van d.V, J. Microsc 1997 1871. doi: 10.1046/j.1365-2818.1997.2410797.x 26. Lakowicz J R Principles of Fluorescence Spectrsocopy2nd edition,edited by Kluwer Academic/Plenum Publishers: New York, Moskow 1999 . doi: null 27. Konig K Riemann I Journal of BiomedicalOptics 2003 8432. doi: 10.1117/1.1577349 28. Lakowicz J R Gryczynski I Arabian Journal for Scienceand Engineering 1992 17261. doi: null 29. Dyba M Klar T A Jakobs S Hell S W AppliedPhysics Letters 2000 77597. doi: 10.1063/1.127056 30. Squire A Verveer P J Bastiaens P I J. Microsc. 2000 197136. doi: 10.1046/j.1365-2818.2000.00651.x 31. Squire J Mueller M Rev. Scientific Instruments 2001 722855. doi: 10.1063/1.1379598 32. Herman P Maliwal B P Lin H J Lakowicz J R J. Microsc. 2001 203176. doi: 10.1046/j.1365-2818.2001.00943.x 33. Bastiaens P I Squire A Trends Cell Biol. 1999 948. doi: 10.1016/S0962-8924(98)01410-X 34. Squire A Bastiaens P I J. Microsc. 1999 19336. doi: 10.1046/j.1365-2818.1999.00427.x 35. Squire A Verveer P J Rocks O Bastiaens P I J. Struct. Biol. 2004 14762. doi: 10.1016/j.jsb.2003.10.013 36. Verveer P J Squire A Bastiaens P I Biophys. J. 2000 782127. doi: null 37. Verveer P J Squire A Bastiaens P I J. Microsc. 2001 202451. doi: 10.1046/j.1365-2818.2001.00917.x 38. Konig K So P T Mantulin W W Tromberg B J Gratton E J Microsc. 1996 183197. doi: null 39. Herman P Maliwal B P Lakowicz J R Anal. Biochem. 2002 30919. doi: 10.1016/S0003-2697(02)00213-0 40. Becker W Bergmann A Biskup C Microsc. Res. Tech. 2007 70403. doi: 10.1002/jemt.20432 41. De B P Owen D M Manning H B Talbot C B Requejo-Isidro J Dunsby C McGinty J Benninger R K Elson D S Munro I John L M Anand P Neil M A French P M Microsc. Res. Tech. 2007 70481. doi: 10.1002/jemt.20434 42. Schonle A Glatz M Hell S W Applied Optics 2000 396306. doi: null 43. Systma J Vroom J M de Grauw C J Gerritsen H C J. Microsc. 1998 19139. doi: 10.1046/j.1365-2818.1998.00351.x 44. van Geest L K Boddeke F R van Dijk P W Kamp A F van der Oord C J R Stoop K W J Proc. SPIE 1999 . doi: null 45. Gerritsen H C Asselbergs M A Agronskaia A V Van Sark W G J. Microsc. 2002 206218. doi: 10.1046/j.1365-2818.2002.01031.x 46. Biskup C Zimmer T Kelbauskas L Hoffmann B Klocker N Becker W Bergmann A Benndorf K Microsc. Res. Tech. 2007 70442. doi: 10.1002/jemt.20431 47. Breusegem S Y Levi M Barry N P Nephron Exp. Nephrol. 2006 103e41. doi: 10.1159/000090615 48. Biskup C Kelbauskas L Zimmer T Benndorf K Bergmann A Becker W Ruppersberg J P Stockklausner C Klocker N J Biomed.Opt. 2004 9753. doi: 10.1117/1.1755721 49. Dowling K Dayel M J Lever M J French P M W Hares J D Dymoke-Bradshaw A K L Optics Letters 1998 23810. doi: null 50. Niesner R Peker B Schlvsche P Gericke K H Chemphyschem. 2004 51141. doi: 10.1002/cphc.200400066 51. Esposito A Gerritsen H C Wouters F S Biophys. J. 2005 894286. doi: 10.1529/biophysj.104.053397 52. Pelet S Previte M J Laiho L H So P T Biophys.J. 2004 872807. doi: 10.1529/biophysj.104.045492 53. Lee K C Siegel J Webb S E Leveque-Fort S Cole M J Jones R Dowling K Lever M J French P M Biophys. J. 2001 811265. doi: null 54. Zhang Z Y Sun T Grattan K T V Palmer A W Proc.SPIE 1997 298090. doi: 10.1117/12.294445 55. Agronskaia A V Tertoolen L Gerritsen H C J Biomed. Opt. 2004 91230. doi: 10.1117/1.1806472 56. Lakowicz J R Szmacinski H Nowaczyk K Johnson M L CellCalcium 1992 13131. doi: 10.1016/0143-4160(92)90041-P 57. Szmacinski H Gryczynski I Lakowicz J R Photochem. Photobiol. 1993 58341. doi: 10.1111/j.1751-1097.1993.tb09571.x 58. Szmacinski H Lakowicz J R Anal. Biochem. 1997 250131. doi: 10.1006/abio.1997.2203 59. Behne M J Meyer J W Hanson K M Barry N P Murata S Crumrine D Clegg R W Gratton E Holleran W M Elias P M Mauro T M J. Biol. Chem. 2002 27747399. doi: 10.1074/jbc.M204759200 60. Behne M J Barry N P Hanson K M Aronchik I Clegg R W Gratton E Feingold K Holleran W M Elias P M Mauro T M J. Invest Dermatol. 2003 120998. doi: 10.1046/j.1523-1747.2003.12262.x 61. Hanson K M Behne M J Barry N P Mauro T M Gratton E Clegg R M Biophys. J. 2002 831682. doi: null 62. Lin H J Herman P Kang J S Lakowicz J R Anal.Biochem. 2001 294118. doi: 10.1006/abio.2001.5155 63. Lin H J Herman P Lakowicz J R Cytometry A 2003 5277. doi: 10.1002/cyto.a.10028 64. Niesner R Peker B Schlusche P Gericke K H Hoffmann C Hahne D Mvller-Goymann C Pharm. Res. 2005 221079. doi: 10.1007/s11095-005-5304-6 65. Stehning C Holst G Proc. SPIE 2001 . doi: null 66. Szmacinski H Castellano F N Terpetschnig E Dattelbaum J D Lakowicz J R Meyer G J Biochim. Biophys. Acta 1998 1383151. doi: null 67. Murtaza Z Herman P Lakowicz J R Biophys. Chem. 1999 80143. doi: 10.1016/S0301-4622(99)00069-1 68. Herman P Murtaza Z Lakowicz J R Anal. Biochem. 1999 27287. doi: 10.1006/abio.1999.4151 69. Chen Y Periasamy A Microsc. Res. Tech. 2004 6372. doi: 10.1002/jemt.10430 70. Elangovan M Day R N Periasamy A Biotechniques 2002 321260. doi: null 71. Elangovan M Day R N Periasamy A J Microsc. 2002 2053. doi: 10.1046/j.0022-2720.2001.00984.x 72. Elangovan M Wallrabe H Chen Y Day R N Barroso M Periasamy A Methods 2003 2958. doi: 10.1016/S1046-2023(02)00283-9 73. Hoefelschweiger B K Pfeifer L Wolfbeis O S Journal of Biomolecular Screening 2005 10687. doi: 10.1177/1087057105277493 74. Jose M Nair D K Reissner C Hartig R Zuschratter W Biophys. J. 2007 922237. doi: 10.1529/biophysj.106.092841 75. Murata S Herman P Lakowicz J R J. Histochem. Cytochem. 2001 491443. doi: null 76. Murata S Herman P Lakowicz J R Cytometry 2001 4394. doi: 10.1002/1097-0320(20010201)43:2<94::AID-CYTO1023>3.0.CO;2-4 77. Murata S Herman P Mochizuki K Nakazawa T Kondo T Nakamura N Lakowicz J R Katoh R J Histochem. Cytochem. 2003 51951. doi: null 78. Murata S Herman P Iwashina M Mochizuki K Nakazawa T Kondo T Nakamura N Lakowicz J R Katoh R J Biomed. Opt. 2005 10034008. doi: 10.1117/1.1924667 79. Nair D K Jose M Kuner T Zuschratter W Hartig R Optics Express 2006 1412217. doi: 10.1364/OE.14.012217 80. Pepperkok R Squire A Geley S Bastiaens P I Curr. Biol. 1999 9269. doi: 10.1016/S0960-9822(99)80117-1 81. Periasamy A Elangovan M Elliott E Brautigan D L Methods Mol. Biol. 2002 18389. doi: null 82. Esposito A Dohm C P Kermer P Bahr M Wouters F S Neurobiol. Dis. 2007 26521. doi: 10.1016/j.nbd.2007.01.014 83. Jia Y Sytnik A Li L Vladimirov S Cooperman B S Hochstrasser R M Proc. Natl. Acad. Sci. USA 1997 947932. doi: 10.1073/pnas.94.15.7932 84. Schuttpelz M Mvller C Neuweiler H Sauer M Anal. Chem. 2006 78663. doi: 10.1021/ac051938j 85. Scully A D Ostler R B Phillips D O'Neill P Townsend K Parker A W MacRobert A J Bioimaging 1997 59. doi: 10.1002/1361-6374(199703)5:1<9::AID-BIO2>3.3.CO;2-1 86. Treanor B Lanigan P M Suhling K Schreiber T Munro I Neil M A Phillips D Davis D M French P M J. Microsc. 2005 21736. doi: 10.1111/j.0022-2720.2005.01430.x 87. Barzda V de Grauw C J Vroom J Kleima F J van G R van A H Gerritsen H C Biophys. J. 2001 81538. doi: null 88. Evans N D Gnudi L Rolinski O J Birch D J Pickup J C Diabetes Technol. Ther. 2003 5807. doi: 10.1089/152091503322527012 89. Lakowicz J R Szmacinski H Nowaczyk K Johnson M L Proceedingsof the National Academy of Sciences of the United States of America 1992 891271. doi: 10.1073/pnas.89.4.1271 90. Schweitzer D Schenke S Hammer M Schweitzer F Jentsch S Birckner E Becker W Bergmann A Microsc. Res. Tech. 2007 70410. doi: 10.1002/jemt.20427 91. Esposito A Gerritsen H C Oggier T Lustenberger F Wouters F S J. Biomed. Opt. 2006 1134016. doi: 10.1117/1.2208999 92. Esposito A Dohm C P Bahr M Wouters F S Mol.Cell Proteomics 2007 . doi: null 93. Evans N D Gnudi L Rolinski O J Birch D J Pickup J C J. Photochem. Photobiol. B 2005 80122. doi: 10.1016/j.jphotobiol.2005.04.001 94. Halbhuber K J König K Annals of Anatomy-AnatomischerAnzeiger 2003 1851. doi: 10.1016/S0940-9602(03)80002-X 95. Pfeifer L Stein K Fink U Welker A Wetzl B Bastian P Wolfbeis O S Journal of Fluorescence 2005 15423. doi: 10.1007/s10895-005-2634-z 96. Tadrous P J Siegel J French P M Shousha S Lalani e Stamp G W J. Pathol. 2003 199309. doi: 10.1002/path.1286 97. Huang S Heikal A A Webb W W Biophys J. 2002 822811. doi: null 98. Benninger R K Onfelt B Neil M A Davis D M French P M Biophys. J. 2005 88609. doi: 10.1529/biophysj.104.050096 99. McClain W M Journal of Chemical Physics 1972 572264. doi: 10.1063/1.1678579
AI Summary 中Eng×
Note: Please note that the content below is AI-generated. Frontiers Journals website shall not be held liable for any consequences associated with the use of this content.