Dopamine-mimetic-coated polyamidoamine-functionalized Fe₃O₄ nanoparticles for safe and efficient gene delivery

Abstract

Fe₃O₄ nanoparticles (NPs) are widely used in the construction of drug and gene delivery vectors because of their particular physicochemical properties. Surface modification can not only reduce the cytotoxicity of Fe₃O₄, but also further improve the biocompatibility and delivery efficiency. In this work, firstly, polydopamine (PDA)-coated Fe₃O₄ NPs (named Fe₃O₄@PDA) were prepared by using the self-polymerization characteristics of dopamine in alkaline environment. Then, polyamidoamine (PAMAM) was modified by the Michael addition reaction to prepare water-soluble core‒shell magnetic NPs of Fe₃O₄@PDA@PAMAM, and its potential as gene vector was further evaluated. The results revealed that Fe₃O₄@PDA@PAMAM had the ability to condense and protect DNA, and showed lower cytotoxicity, higher cell uptake and transfection efficiency than those of PAMAM. It has the potential to become a magnetic targeted gene vector for further study.

Graphical abstract

Keywords

gene vector / nanoparticle / polydopamine / gene transfection

References

[1] Yahya E B, Alqadhi A M . Recent trends in cancer therapy: a review on the current state of gene delivery.Life Sciences, 2021, 269: 119087

[2] Urban D L, Schneider I . Gene-specific treatment approaches in muscle diseases.Der Nervenarzt, 2020, 91(4): 318–323

[3] Fox T A, Booth C . Gene therapy for primary immunodeficiencies.British Journal of Haematology, 2021, 193(6): 1044–1059

[4] Blanco E, Izotova N, Booth C, . Immune reconstitution after gene therapy approaches in patients with X-linked severe combined immunodeficiency disease.Frontiers in Immunology, 2020, 11: 608653

[5] Chu Y P, Bartus R T, Manfredsson F P, . Long-term post-mortem studies following neurturin gene therapy in patients with advanced Parkinson’s disease.Brain, 2020, 143: 960–975

[6] Huang N C, Lee C M, Hsu S H . Effective naked plasmid DNA delivery into stem cells by microextrusion-based transient-transfection system for in situ cardiac repair.Cytotherapy, 2020, 22(2): 70–81

[7] Mohammadinejad R, Dehshahri A, Madamsetty V S, . In vivo gene delivery mediated by non-viral vectors for cancer therapy.Journal of Controlled Release, 2020, 325: 249–275

[8] Kashkouli K I, Torkzadeh-Mahani M, Mosaddegh E . Synthesis and characterization of aminotetrazole-functionalized magnetic chitosan nanocomposite as a novel nanocarrier for targeted gene delivery.Materials Science and Engineering C: Materials for Biological Applications, 2018, 89: 166–174

[9] Chan Y K, Wang S K, Chu C J, . Engineering adeno-associated viral vectors to evade innate immune and inflammatory responses.Science Translational Medicine, 2021, 13(580): eabd3438

[10] Zhao Z M, Anselmo A C, Mitragotri S . Viral vector-based gene therapies in the clinic.Bioengineering & Translational Medicine, 2022, 7(1): e10258

[11] Hromic-Jahjefendic A, Lundstrom K . Viral vector-based melanoma gene therapy.Biomedicines, 2020, 8(3): 60

[12] Zu H, Gao D C . Non-viral vectors in gene therapy: recent development, challenges, and prospects.AAPS Journal, 2021, 23(4): 78

[13] Vemana H P, Saraswat A, Bhutkar S, . A novel gene therapy for neurodegenerative Lafora disease via EPM2A-loaded DLinDMA lipoplexes.Nanomedicine, 2021, 16(13): 1081–1095

[14] Jerzykiewicz J, Czogalla A . Polyethyleneimine-based lipopolyplexes as carriers in anticancer gene therapies.Materials, 2022, 15(1): 179

[15] Guo Z S, Lu B F, Guo Z B, . Vaccinia virus-mediated cancer immunotherapy: cancer vaccines and oncolytics.Journal for Immunotherapy of Cancer, 2019, 7: 6

[16] Ravula V, Lo Y L, Wang L F, . Gemini lipopeptide bearing an ultrashort peptide for enhanced transfection efficiency and cancer-cell-specific cytotoxicity.ACS Omega, 2021, 6(35): 22955–22968

[17] Petithory T, Pieuchot L, Josien L, . Size-dependent internalization efficiency of macrophages from adsorbed nanoparticle-based monolayers.Nanomaterials, 2021, 11(8): 1963

[18] Pontes J F, Grenha A . Multifunctional nanocarriers for lung drug delivery.Nanomaterials, 2020, 10(2): 183

[19] Khalil M, Haq E A, Dwiranti A, . Bifunctional folic-conjugated aspartic-modified Fe₃O₄ nanocarriers for efficient targeted anticancer drug delivery.RSC Advances, 2022, 12(8): 4961–4971

[20] Goswami B, Mahanta D . Polyaniline‒Fe₃O₄ and polypyrrole‒Fe₃O₄ magnetic nanocomposites for removal of 2, 4-dichlorophenoxyacetic acid from aqueous medium.Journal of Environmental Chemical Engineering, 2020, 8(4): 103913

[21] Dutta B, Nema A, Shetake N G, . Glutamic acid-coated Fe₃O₄ nanoparticles for tumor-targeted imaging and therapeutics.Materials Science and Engineering C: Materials for Biological Applications, 2020, 112: 110915

[22] Kao Y T, Chen Y T, Fan H C, . Novel coagulation factor VIII gene therapy in a mouse model of hemophilia a by lipid-coated Fe₃O₄ nanoparticles.Biomedicines, 2021, 9(9): 1116

[23] Wang Y H, Wu S Q, Yin Q J, . Novel hybrid p- and n-type organic thermoelectric materials based on mussel-inspired polydopamine.ACS Applied Materials & Interfaces, 2021, 13(20): 23970–23982

[24] Demirci S, Sahiner M, Suner S S, . Improved biomedical properties of polydopamine-coated carbon nanotubes.Micromachines, 2021, 12(11): 1280

[25] Deng H, Yang Y M, Tang X H, . Phase-change composites composed of silicone rubber and Pa@SiO₂@PDA double-shelled microcapsules with low leakage rate and improved mechanical strength.ACS Applied Materials & Interfaces, 2021, 13(33): 39394–39403

[26] Tian M Y, Xin F H, Gao Y J, . Design of a near-infrared-triggered photo/thermal dual-responsive composite carrier with excellent biocompatibility for controllable drug release.Journal of Applied Polymer Science, 2022, 139(17): e52029

[27] Hu Y, Dan W H, Xiong S B, . Development of collagen/polydopamine complexed matrix as mechanically enhanced and highly biocompatible semi-natural tissue engineering scaffold.Acta Biomaterialia, 2017, 47: 135–148

[28] Hossein-Beigi F, Fatahian S, Shahbazi-Gahrouei S, . Assessment of ploy dopamine coated Fe₃O₄ nanoparticles for melanoma (B16-F10 and A-375) cells detection.Anti-cancer Agents in Medicinal Chemistry, 2020, 20(16): 1918–1926

[29] Yang X, Niu X H, Mo Z L, . Electrochemical chiral interface based on the Michael addition/Schiff base reaction of polydopamine functionalized reduced graphene oxide.Electrochimica Acta, 2019, 319: 705–715

[30] Lu C C, Li Z Q, Chang L, . Efficient delivery of dsRNA and DNA in cultured silkworm cells for gene function analysis using PAMAM dendrimers system.Insects, 2020, 11(1): 12

[31] Wang D, Wang J, Song J, . Guanidyl and imidazolyl integration group-modified PAMAM for gastric adenocarcinoma gene therapy.The Journal of Gene Medicine, 2020, 22(10): e3240

[32] Melgar-Lesmes P, Luquero A, Parra-Robert M, . Graphene-dendrimer nanostars for targeted macrophage overexpression of metalloproteinase 9 and hepatic fibrosis precision therapy.Nano Letters, 2018, 18(9): 5839–5845

[33] de Albuquerque Y L, Berger E, Tomaz S, . Evaluation of the toxicity on lung cells of by-products present in naphthalene secondary organic aerosols.Life, 2021, 11(4): 319

[34] Lebourgeois S, Fraisse A, Hennechart-Collette C, . Development of a real-time cell analysis (RTCA) method as a fast and accurate method for detecting infectious particles of the adapted strain of hepatitis a virus.Frontiers in Cellular and Infection Microbiology, 2018, 8: 335

[35] Hazekawa M, Nishinakagawa T, Kawakubo-Yasukochi T, . Evaluation of IC50 levels immediately after treatment with anticancer reagents using a real-time cell monitoring device.Experimental and Therapeutic Medicine, 2019, 18(4): 3197–3205

[36] Sun Y M, Li D Y, Sun Y K, . Effect of dopamine dosages on the microstructure and adsorption properties of the Fe₃O₄@PDA@PAMAM magnetic nano-adsorbed materials.Chinese Journal of Inorganic Chemistry, 2020, 36(2): 283–288

[37] Chen G P, Zhang G F, Yang F C . The elaboration of multifunctional hollow core‒shell Fe₃O₄@PDA@TiO₂ architecture with dual magnetic- and photo-responsive performance.New Journal of Chemistry, 2020, 44(8): 3487–3492

[38] Liu S J, Fu J W, Wang M H, . Magnetically separable and recyclable Fe₃O₄‒polydopamine hybrid hollow microsphere for highly efficient peroxidase mimetic catalysts.Journal of Colloid and Interface Science, 2016, 469: 69–77

[39] Wang J J, Gong C, Wang Y N, . Magnetic nanoparticles with a pH-sheddable layer for antitumor drug delivery.Colloids and Surfaces B: Biointerfaces, 2014, 118: 218–225

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