Regulation effect of osteoblasts towards osteocytes by silk fibroin encapsulation

Abstract

Herein, the rational design micromilieus involved silk fibroin (SF)-based materials have been used to encapsulate the osteoblasts, forming an extracellular coated shell on the cells, which exhibited the high potential to shift the regulation of osteoblasts to osteocytes by encapsulation cues. SF coating treated cells showed a change in cell morphology from osteoblasts-like to osteocytes-like shape compared with untreated ones. Moreover, the expression of alkaline phosphatase (ALP), collagen I (Col I) and osteocalcin (OCN) further indicated a potential approach for inducing osteoblasts regulation, which typically accelerates calcium deposition and cell calcification, presenting a key role for the SF encapsulation in controlling osteoblasts behavior. This discovery showed that SF-based cell encapsulation could be used for osteoblasts behavior regulation, which offers a great potential to modulate mammalian cells’ phenotype involving alternating surrounding cues.

Graphical abstract

Keywords

cell encapsulation / silk fibroin / osteoblasts modulation / cell differentiation / cell calcification

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Disclosure of potential conflicts of interests

The authors declare that they have no conflict of interest.

Acknowledgements

This research was financially supported by the National Natural Science Foundation of China (Grant Nos. 51902289 and 51672250), the Natural Science Foundation of Zhejiang Province (Grant No. LQ19E020010), the Project of Zhejiang Provincial Department of Education (Grant No. Y201840323), the Key Project of Zhejiang Science and Technology (Grant Nos. 2021C01180 and 2019C04020), and the Research Foundation of ZSTU (Grant Nos. 18012134-Y and 2020Q008).

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