Injectable hydrogel wound dressing based on strontium ion cross-linked starch

Abstract

Severe skin wounds cause great problems and sufferings to patients. In this study, an injectable wound dressing based on strontium ion cross-linked starch hydrogel (SSH) was developed and evaluated. The good inject-ability of SSH made it easy to be delivered onto the wound surface. The good tissue adhesiveness of SSH ensured a firm protection of the wound. Besides, SSH supported the proliferation of NIH/3T3 fibroblasts and facilitated the migration of human umbilical vein endothelial cells (HUVECs). Importantly, SSH exhibited strong antibacterial effects on Staphylococcus aureus (S. aureus), which could prevent wound infection. These results demonstrate that SSH is a promising wound dressing material for promoting wound healing.

Keywords

wound dressing / injectable / antibacterial / gel-point adhesive hydrogel (GPAH)

References

[1] Micallef G, Bickerdike R, Reiff C, . Exploring the transcriptome of Atlantic salmon (Salmo salar) skin, a major defense organ. Marine Biotechnology, 2012, 14(5): 559–569

[2] Strese H, Kuck M, Benken R, . Application of optical methods to characterize textile materials and their influence on the human skin. Journal of Biomedical Optics, 2011, 16(4): 046013

[3] Xu R, Luo G, Xia H, . Novel bilayer wound dressing composed of silicone rubber with particular micropores enhanced wound re-epithelialization and contraction. Biomaterials, 2015, 40: 1–11

[4] Peck M D. Epidemiology of burns throughout the world. Part I: Distribution and risk factors. Burns, 2011, 37(7): 1087–1100

[5] Muhammad A A, Arulselvan P, Cheah P S, . Evaluation of wound healing properties of bioactive aqueous fraction from Moringa oleifera Lam on experimentally induced diabetic animal model. Drug Design, Development and Therapy, 2016, 10: 1715–1730

[6] Strassburg A, Petranowitsch J, Paetzold F, . Cross-linking of a hydrophilic, antimicrobial polycation toward a fast-swelling, antimicrobial superabsorber and interpenetrating hydrogel networks with long lasting antimicrobial properties. ACS Applied Materials & Interfaces, 2017, 9(42): 36573–36582

[7] Murakami K, Aoki H, Nakamura S, . Hydrogel blends of chitin/chitosan, fucoidan and alginate as healing-impaired wound dressings. Biomaterials, 2010, 31(1): 83–90

[8] Jayakumar R, Prabaharan M, Kumar P T S, . Biomaterials based on chitin and chitosan in wound dressing application. Biotechnology Advances, 2011, 29(3): 322–337

[9] Hasatsri S, Pitiratanaworanat A, Swangwit S, . Comparison of the morphological and physical properties of different absorbent wound dressings. Dermatology Research and Practice, 2018, 9: 9367034

[10] Tamer T M, Valachová K, Hassan M A, . Chitosan/hyaluronan/edaravone membranes for anti-inflammatory wound dressing: In vitro and in vivo evaluation studies. Materials Science and Engineering C, 2018, 90: 227–235

[11] Hassiba A J, Zowalaty M E, Nasrallah G K, . Review of recent research on biomedical applications of electrospun polymer nanofibers for improved wound healing. Nanomedicine, 2016, 11(6): 715–737

[12] Caló E, Khutoryanskiy V V. Biomedical applications of hydrogels: A review of patents and commercial products. European Polymer Journal, 2015, 65(SI): 252–267

[13] Kamoun E A, Kenawy E R S, Chen X. A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings. Journal of Advanced Research, 2017, 8(3): 217–233

[14] Li H, Yang J, Hu X, . Superabsorbent polysaccharide hydrogels based on pullulan derivate as antibacterial release wound dressing. Journal of Biomedical Materials Research Part A, 2011, 98(1): 31–39

[15] Sung J H, Hwang M R, Kim J O, . Gel characterisation and in vivo evaluation of minocycline-loaded wound dressing with enhanced wound healing using polyvinyl alcohol and chitosan. International Journal of Pharmaceutics, 2010, 392(1–2): 232–240

[16] Balakrishnan B, Jayakrishnan A, Kumar S, . Anti-bacterial properties of an in situ forming hydrogel based on oxidized alginate and gelatin loaded with gentamycin. Trends in Biomaterials & Artificial Organs, 2012, 26(3): 139–145

[17] Gong C, Wu Q, Wang Y, . A biodegradable hydrogel system containing curcumin encapsulated in micelles for cutaneous wound healing. Biomaterials, 2013, 34(27): 6377–6387

[18] Li L, Yan B, Yang J, . Novel mussel-inspired injectable self-healing hydrogel with anti-biofouling property. Advanced Mater-ials, 2015, 27(7): 1294–1299

[19] Liu H, Zhang Z, Ge J, . A flexible conductive hybrid elastomer for high-precision stress/strain and humidity detection. Journal of Materials Science and Technology, 2019, 35(1): 176–180

[20] Elvira C, Mano J F, San Roman J, . Starch-based biodegradable hydrogels with potential biomedical applications as drug delivery systems. Biomaterials, 2002, 23(9): 1955–1966

[21] Ismail H, Irani M, Ahmad Z. Starch-based hydrogels: present status and applications. International Journal of Polymeric Materials and Polymeric Biomaterials, 2013, 62(7): 411–420

[22] Zhao F, Lei B, Li X, . Promoting in vivo early angiogenesis with sub-micrometer strontium-contained bioactive microspheres through modulating macrophage phenotypes. Biomaterials, 2018, 178: 36–47

[23] Gu Z P, Xie H X, Li L, . Application of strontium-doped calcium polyphosphate scaffold on angiogenesis for bone tissue engineering. Journal of Materials Science: Materials in Medicine, 2013, 24(5): 1251–1260

[24] Rajeswari D, Gopi D, Ramya S, . Investigation of anti-corrosive, antibacterial and in vitro biological properties of a sul-phonated poly(etheretherketone)/strontium, cerium co-substituted hydroxyapatite composite coating developed on surface treated surgical grade stainless steel for orthopedic applications. RSC Advances, 2014, 4(106): 61525–61536

[25] Gao C, Liu H, Luo Z P, . Modification of calcium phosphate cement with poly (γ-glutamic acid) and its strontium salt for kyphoplasty application. Materials Science and Engineering C, 2017, 80: 352–361

[26] Taggart P. Starch as an ingredient: manufacture and applications. In: Eliasson A C, ed. Starch in Food: Structure, Functions and Applications. Cambridge, UK: Woodhead Publishing Ltd., 2004, 363–392

[27] Lin X, Liu Y, Bai A, . A viscoelastic adhesive epicardial patch for treating myocardial infarction. Nature Biomedical Engineering, 2019, 3(8): 632–643

[28] Mehdizadeh M, Weng H, Gyawali D, . Injectable citrate-based mussel-inspired tissue bioadhesives with high wet strength for sutureless wound closure. Biomaterials, 2012, 33: 7972–7983

[29] Guo J, Kim G B, Shan D, . Click chemistry improved wet adhesion strength of mussel-inspired citrate-based antimicrobial bioadhesives. Biomaterials, 2017, 112: 275–286

[30] Liu L, Liu Y Q, Feng C, . Lithium-containing biomaterials stimulate bone marrow stromal cell-derived exosomal miR-130a secretion to promote angiogenesis. Biomaterials, 2019, 192: 523–536

[31] Ciesielski W, Tomasik P. Complexes of amylose and amylopectins with multivalent metal salts. Journal of Inorganic Bioche-mistry, 2004, 98(12): 2039–2051

[32] Tomasik P, Schilling C H. Complexes of starch with inorganic guests. Advances in Carbohydrate Chemistry and Biochemistry, 1998, 53: 263–343

[33] Qu J, Zhao X, Liang Y, . Antibacterial adhesive injectable hydrogels with rapid self-healing, extensibility and compressibility as wound dressing for joints skin wound healing. Biomaterials, 2018, 183: 185–199

[34] Faghihnejad A, Feldman K E, Yu J, . Adhesion and surface interactions of a self-healing polymer with multiple hydrogen-bonding groups. Advanced Functional Materials, 2014, 24(16): 2322–2333

[35] Hofman A H, van Hees I A, Yang J, . Bioinspired underwater adhesives by using the supramolecular toolbox. Advanced Materials, 2018, 30(19): 1704640

[36] Peak C W, Wilker J J, Schmidt G. A review on tough and sticky hydrogels. Colloid and Polymer Science, 2013, 291(9): 2031–2047

[37] Karami P, Wyss C S, Khoushabi A, . Composite double-network hydrogels to improve adhesion on biological surfaces. ACS Applied Materials & Interfaces, 2018, 10(45): 38692–38699

[38] Martina D, Creton C, Damman P, . Adhesion of soft viscoelastic adhesives on periodic rough surfaces. Soft Matter, 2012, 8(19): 5350–5357

[39] Shazly T M, Artzi N, Boehning F, . Viscoelastic adhesive mechanics of aldehyde-mediated soft tissue sealants. Biomaterials, 2008, 29(35): 4584–4591

[40] Saidak Z, Marie P J. Strontium signaling: Molecular mechanisms and therapeutic implications in osteoporosis. Pharmacology & Therapeutics, 2012, 136(2): 216–226

[41] Bikkavilli R K, Feigin M E, Malbon C C. p38 mitogen-activated protein kinase regulates canonical Wnt-β-catenin signaling by inactivation of GSK3β. Journal of Cell Science, 2008, 121(21): 3598–3607

[42] Kamath K R, Park K. Biodegradable hydrogels in drug delivery. Advanced Drug Delivery Reviews, 1993, 11(1–2): 59–84

[43] Ende N. Amylase activity in body fluids. Cancer, 1961, 14(5): 1109–1114

[44] Lamalice L, Le Boeuf F, Huot J. Endothelial cell migration during angiogenesis. Circulation Research, 2007, 100(6): 782–794

[45] Gao Z X Z, Huang D Y, Li H X, . Scutellarin promotes in vitro angiogenesis in human umbilical vein endothelial cells. Biochemical and Biophysical Research Communications, 2010, 400(1): 151–156

[46] Li S, Li L, Guo C, . A promising wound dressing material with excellent cytocompatibility and proangiogenesis action for wound healing: Strontium loaded silk fibroin/sodium alginate (SF/SA) blend films. International Journal of Biological Macromolecules, 2017, 104: 969–978

[47] Atiyeh B S, Costagliola M, Hayek S N, . Effect of silver on burn wound infection control and healing: Review of the literature. Burns, 2007, 33(2): 139–148

[48] Konvalinka A, Errett L, Fong I W. Impact of treating Staphylococcus aureus nasal carriers on wound infections in cardiac surgery. Journal of Hospital Infection, 2006, 64(2): 162–168

[49] Guida A, Towler M R, Wall J G, . Preliminary work on the antibacterial effect of strontium in glass ionomer cements. Journal of Materials Science Letters, 2003, 22(20): 1401–1403

[50] Lundberg J O, Weitzberg E, Gladwin M T. The nitrate–nitrite–nitric oxide pathway in physiology and therapeutics. Nature Reviews Drug Discovery, 2008, 7(2): 156–167

[51] Ravi N D, Balu R, Kumar T S S. Strontium-substituted calcium deficient hydroxyapatite nanoparticles: synthesis, characterization, and antibacterial properties. Journal of the American Ceramic Society, 2012, 95(9): 2700–2708

Acknowledgements

This work was sponsored by the National Natural Science Foundation of China (Grant Nos. 51672184, 81930070, 81622032 and 81501858), the Natural Science Research of Jiangsu Higher Education Institutions (No. 17KJA180011), the Jiangsu Innovation and Entrepreneurship Program, and the Priority Academic Program Development of Jiangsu High Education Institutions (PAPD).

RIGHTS & PERMISSIONS

2020 Higher Education Press