The comparison of the Wnt signaling pathway inhibitor delivered electrospun nanoyarn fabricated with two methods for the application of urethroplasty

Abstract

Urethral strictures were common disease caused by over-expression of extracellular matrix from fibroblast. In this study, we compare two nanoyarn scaffolds for improving fibroblasts infiltration without inhibition the over-expression of extracellular matrix. Collagen/poly(L-lactide-co-caprolactone) (Col/P(LLA-CL)) nanoyarn scaffolds were prepared by conjugated electrospinning and dynamic liquid electrospinning, respectively. In addition, co-axial electrospinning technique was combined with the nanoyarn fabrication process to produce nanoyarn scaffolds loading Wnt signaling pathway inhibitor. The mechanical properties of the scaffolds were examined and morphology was observed by SEM. Cell morphology, proliferation and infiltration on the scaffolds were investigated by SEM, MTT assay and H&E staining, respectively. The release profiles of different scaffolds were determined using HPLC. The results indicated that cells showed an organized morphology along the nanoyarns and considerable infiltration into the nanoyarn scaffolds prepared by dynamic liquid electrospinning (DLY). It was also observed that the DLY significantly facilitate cell proliferation. The D-DLY could facilitate the infiltration of the fibroblasts and could be a promising scaffold for the treatment of urethra stricture while it may inhibit the collagen production.

Keywords

biomaterials / nanoyarn / electrospinning / inhibitor

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Disclosure of potential conflicts of interests

There is no conflict of interest among the authors.

Acknowledgements

The study was supported by the National Natural Science Foundation of China (Grant Nos. 31470941 and 31271035), the Science and Technology Commission of Shanghai Municipality (Grant Nos. 14JC1492100, 15JC1490100 and 15441905100), the Yantai Double Hundred Talent Plan and the Ph.D. Programs Foundation of Ministry of Education of China (Grant No. 20130075110005) and light of textile project (Grant No. J201404). The authors extend their appreciation to the International Scientific Partnership Program ISPP at King Saud University for its funding research through ISPP-0049.

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2016 Higher Education Press and Springer-Verlag Berlin Heidelberg