Electrophoretic-deposited novel ternary silk fibroin/graphene oxide/hydroxyapatite nanocomposite coatings on titanium substrate for orthopedic applications

Abstract

The combination of graphene oxide (GO) with robust mechanical property, silk fibroin (SF) with fascinating biological effects and hydroxyapatite (HA) with superior osteogenic activity is a competitive approach to make novel coatings for orthopedic applications. Herein, the feasibility of depositing ternary SF/GO/HA nanocomposite coatings on Ti substrate was firstly verified by exploiting electrophoretic nanotechnology, with SF being used as both a charging additive and a dispersion agent. The surface morphology, microstructure and composition, in vitro hemocompatibility and in vitro cytocompatibility of the resulting coatings were investigated by SEM, Raman, FTIR spectra and biocompatibility tests. Results demonstrated that GO, HA and SF could be co-deposited with a uniform, smooth thin-film morphology. The hemolysis rate analysis and the platelet adhesion test indicated good blood compatibility of the coatings. The human osteosarcoma MG63 cells displayed well adhesion and proliferation behaviors on the prepared coatings, with enhanced ALP activities. The present study suggested that SF/GO/HA nanocomposite coatings could be a promising candidate for the surface functionalization of biomaterials, especially as orthopedic implant coating.

Keywords

graphene oxide / silk fibroin / hydroxyapatite / nano-biocomposite coatings / electrodeposition

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Acknowledgements

This work was supported by the Beijing Natural Science Foundation (Grant No. 2164073), the National Natural Science Foundation of China (Grant Nos. 21271032, 31370954 and 51431002), the Scientific and Technical Program of Beijing (Grant No. Z141100002814008), and the Innovative-Talent Program (Institute of Electrical Engineering, Chinese Academy of Sciences).

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2016 Higher Education Press and Springer-Verlag Berlin Heidelberg