Evaluation on biocompatibility of biomedical polyurethanes with different hard segment contents

Abstract

In this paper, polyurethane (PU) materials with different contents of hard segment (20%, 25%, 30%) were prepared based on hexamethylene diisocyanate and polycarbonate diols by solution polymerization. The obtained polycarbonate-urethane (PCU) elastomers were characterized by very good hydrophobic property and excellent resistance to hydrolysis. Hemolysis, recalification time and platelet-rich plasma adhesion were used to evaluate the blood compatibility of the materials. L929 cells cultured with leach liquor of these PU membranes were selected to perform the cytotoxicity experiments. The results indicate that the hemolysis rates of PU membranes are all less than 5%, which can meet the requirement of the national standards for biomaterials. However, compared with 20% and 30% groups, the recalification time of the sample containing 25% hard segment is longer, while the number of platelet adhesion is less. Additionally, cells cultured in the leach liquor of PU membranes with 25% hard segment proliferated relatively more thriving, meaning that this proportion of the material has the lowest cytotoxicity.

Keywords

polyurethane (PU) / hydrolytic stability / blood compatibility / cytotoxicity

References

[1] Chen L, Han D, Jiang L. On improving blood compatibility: from bioinspired to synthetic design and fabrication of biointerfacial topography at micro/nano scales. Colloids and Surfaces B: Biointerfaces, 2011, 85(1): 2–7

[2] Ratner B D. The catastrophe revisited: blood compatibility in the 21st Century. Biomaterials, 2007, 28(34): 5144–5147

[3] Gorbet M B, Sefton M V. Biomaterial-associated thrombosis: roles of coagulation factors, complement, platelets and leukocytes. Biomaterials, 2004, 25(26): 5681–5703

[4] Mao C, Qiu Y, Sang H, . Various approaches to modify biomaterial surfaces for improving hemocompatibility. Advances in Colloid and Interface Science, 2004, 110(1−2): 5–17

[5] Szycher M, Siciliano A A, Reed A M. Polyurethane elastomers in medicine. Polymeric. Biomaterials. New York: Marcel Dekker, 1994, 233–244

[6] Simmons A, Hyvarinen J, Odell R A, . Long-term in vivo biostability of poly(dimethylsiloxane)/poly(hexamethylene oxide) mixed macrodiol-based polyurethane elastomers. Biomaterials, 2004, 25(20): 4887–4900

[7] Baumann H, Richter A, Klemm D, . Concepts for preparation of novel regioselective modified cellulose derivatives sulfated, aminated, carboxylated and acetylated for hemocompatible ultrathing coatings on biomaterials. Macromolecular Chemistry and Physics, 2000, 201(15): 1950–1962

[8] Bernacca G M, Gulbransen M J, Wilkinson R, . In vitro blood compatibility of surface-modified polyurethanes. Biomaterials, 1998, 19(13): 1151–1165

[9] Jo H Y, Jung W K, Kim S K. Purification and characterization of a novel anticoagulant peptide from marine echiuroid worm, Urechis unicinctus. Process Biochemistry, 2008, 43(2): 179–184

[10] Laredo J, Xue L, Husak V A, . Silyl-heparin bonding improves the patency and in vivo thromboresistance of carbon-coated polytetrafluoroethylene vascular grafts. Journal of Vascular Surgery, 2004, 39(5): 1059–1065

[11] Sawada S, Iwasaki Y, Nakabayashi N, . Stress response of adherent cells on a polymer blend surface composed of a segmented polyurethane and MPC copolymers. Journal of Biomedical Materials Research Part A, 2006, 79(3): 476–484

[12] Jia R P, Zong A X, He X Y, . Synthesis of newly fluorinated thermoplastic polyurethae elastomers and theirblood compatibility. Fibers and Polymers, 2015, 16(2): 231–238

[13] Yan X, Chen J, Yang J, . Fabrication of free-standing, electrochemically active, and biocompatible graphene oxide-polyaniline and graphene-polyaniline hybrid papers. ACS Applied Materials & Interfaces, 2010, 2(9): 2521–2529

[14] Fang H, Wei J, Yu Y. In vivo studies of endotoxin removal by lysine-cellulose adsorbents. Biomaterials, 2004, 25(23): 5433–5440

[15] Kuran W, Sobczak M, Listos T, . New route to oligocarbonate diols suitable for the synthesis of polyurethane elastomers. Polymer, 2000, 41(24): 8531–8541

[16] Zhang J, Hu C P. Synthesis, characterization and mechanical properties of polyester-based aliphatic polyurethane elastomers containing hyperbranched polyester segments. European Polymer Journal, 2008, 44(11): 3708–3714

[17] Sagnella S, Mai-Ngam K. Chitosan based surfactant polymers designed to improve blood compatibility on biomaterials. Colloids and Surfaces B: Biointerfaces, 2005, 42(2): 147–155

[18] American Society for Testing and Materials. ASTM F 756-00, Standard Practices for Assessment of Haemolytic Properties of Materials, 2000

[19] Xue J, Zhao W, Nie S, . Blood compatibility of polyethersulfone membrane by blending a sulfated derivative of chitosan. Carbohydrate Polymers, 2013, 95(1): 64–71

[20] Yuse K, Guiffard B, Belouadah R, . Polymer nanocomposites for microactuation and magneto-electric transduction. Frontiers of Mechanical Engineering in China, 2009, 4(3): 350–354

Acknowledgements

This work was supported by the Hong Kong, Macao and Taiwan Science & Technology Cooperation Program of China (Grant No. 2015DFH30180), the Key Grant Project of Chinese Ministry of Education (Grant No. 313041), the Key Technology Research Plan of Wuhan Municipality (Grant No. 2014060202010120), and the Fundamental Research Funds for the Central Universities (WUT: 2014-VII-028).

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg