Remineralization of initial enamel caries in vitro using a novel peptide based on amelogenin

Abstract

Dental caries is the most common oral disease with high incidence, widely spread and can seriously affect the health of oral cavity and the whole body. Current caries prevention measures such as fluoride treatment, antimicrobial agents, and traditional Chinese herbal, have limitations to some extent. Here we design and synthesize a novel peptide based on the amelogenin, and assess its ability to promote the remineralization of initial enamel caries lesions. We used enamel blocks to form initial lesions, and then subjected to 12-day pH cycling in the presence of peptide, NaF and HEPES buffer. Enamel treated with peptide or NaF had shallower, narrower lesions, thicker remineralized surfaces and less mineral loss than enamel treated with HEPES. This peptide can promote the remineralization of initial enamel caries and inhibit the progress of caries. It is a promising anti-caries agent with various research prospects and practical application value.

Keywords

dental caries / peptide / remineralization

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Disclosure of potential conflicts of interests

We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with this work published.

This work was supported by the National Natural Science Foundation of China (Grant Nos. 81000431 and 81271128) and the New Century Excellent Talents University Support Programme. In addition we thank the Crest Research Laboratory of Procter & Gamble Technology (Beijing) Co. Ltd. for help with the transverse microradiography analyses.

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg