Remineralization of initial enamel caries in vitro using a novel peptide based on amelogenin

Danxue LI, Xueping LV, Huanxin TU, Xuedong ZHOU, Haiyang YU, Linglin ZHANG

PDF(5079 KB)
PDF(5079 KB)
Front. Mater. Sci. ›› 2015, Vol. 9 ›› Issue (3) : 293-302. DOI: 10.1007/s11706-015-0298-4
RESEARCH ARTICLE

Remineralization of initial enamel caries in vitro using a novel peptide based on amelogenin

Author information +
History +

Abstract

Dental caries is the most common oral disease with high incidence, widely spread and can seriously affect the health of oral cavity and the whole body. Current caries prevention measures such as fluoride treatment, antimicrobial agents, and traditional Chinese herbal, have limitations to some extent. Here we design and synthesize a novel peptide based on the amelogenin, and assess its ability to promote the remineralization of initial enamel caries lesions. We used enamel blocks to form initial lesions, and then subjected to 12-day pH cycling in the presence of peptide, NaF and HEPES buffer. Enamel treated with peptide or NaF had shallower, narrower lesions, thicker remineralized surfaces and less mineral loss than enamel treated with HEPES. This peptide can promote the remineralization of initial enamel caries and inhibit the progress of caries. It is a promising anti-caries agent with various research prospects and practical application value.

Keywords

dental caries / peptide / remineralization

Cite this article

Download citation ▾
Danxue LI, Xueping LV, Huanxin TU, Xuedong ZHOU, Haiyang YU, Linglin ZHANG. Remineralization of initial enamel caries in vitro using a novel peptide based on amelogenin. Front. Mater. Sci., 2015, 9(3): 293‒302 https://doi.org/10.1007/s11706-015-0298-4

References

[1]
Brambilla E. Fluoride- is it capable of fighting old and new dental diseases? An overview of existing fluoride compounds and their clinical applications. Caries Research, 2001, 35(Suppl 1): 6–9
[2]
Sheng J, Liu Z. Induction of fluoride-resistant mutant of S. mutans and the measurement of its acidogenesis in vitro. Chinese Journal of Stomatological Research, 2000, 35(2): 95–98 (in Chinese)
[3]
Li L. The biochemistry and physiology of metallic fluoride: action, mechanism, and implications. Critical Reviews in Oral Biology and Medicine, 2003, 14(2): 100–114
[4]
Dickens S H, Flaim G M, Takagi S. Mechanical properties and biochemical activity of remineralizing resin-based Ca-PO4 cements. Dental Materials, 2003, 19(6): 558–566
[5]
Van Loveren C. Sugar alcohols: what is the evidence for caries-preventive and caries-therapeutic effects? Caries Research, 2004, 38(3): 286–293
[6]
Wang X J, Huang H, Yang F, . Ectopic study of tissue-engineered bone complex with enamel matrix proteins, bone marrow stromal cells in porous calcium phosphate cement scaffolds, in nude mice. Cell Proliferation, 2011, 44(3): 274–282
[7]
Mrozik K M, Gronthos S, Menicanin D, . Effect of coating Straumann® Bone Ceramic with Emdogain on mesenchymal stromal cell hard tissue formation. Clinical Oral Investigations, 2012, 16(3): 867–878
[8]
Rathe F, Junker R, Chesnutt B M, . The effect of enamel matrix derivative (Emdogain) on bone formation: a systematic review. Tissue Engineering Part B: Reviews, 2009, 15(3): 215–224
[9]
Suzuki S, Nagano T, Yamakoshi Y, . Enamel matrix derivative gel stimulates signal transduction of BMP and TGF-β. Journal of Dental Research, 2005, 84(6): 510–514
[10]
Windisch P, Sculean A, Klein F, . Comparison of clinical, radiographic, and histometric measurements following treatment with guided tissue regeneration or enamel matrix proteins in human periodontal defects. Journal of Periodontology, 2002, 73(4): 409–417
[11]
Zhang L, Zou L, Li J, . Effect of enamel organic matrix on the potential of Galla chinensis to promote the remineralization of initial enamel carious lesions in vitro. Biomedical Materials, 2009, 4(3): 034102
[12]
Zhang L, Xue J, Li J, . Effects of Galla chinensis on inhibition of demineralization of regular bovine enamel or enamel disposed of organic matrix. Archives of Oral Biology, 2009, 54(9): 817–822
[13]
Chen H, Clarkson B H, Sun K, . Self-assembly of synthetic hydroxyapatite nanorods into an enamel prism-like structure. Journal of Colloid and Interface Science, 2005, 288(1): 97–103
[14]
Yamagishi K, Onuma K, Suzuki T, . Materials chemistry: a synthetic enamel for rapid tooth repair. Nature, 2005, 433(7028): 819
[15]
Wang Z W, Zhao Y P, Zhou C R, . The study on the enamel remineralization by enamel matrix proteins’ inducing. Journal of Sichuan University (Medical Science Edition), 2008, 39(4): 579–582 (in Chinese)
[16]
Ishizaki N T, Matsumoto K, Kimura Y, . Histopathological study of dental pulp tissue capped with enamel matrix derivative. Journal of Endodontics, 2003, 29(3): 176–179
[17]
Xiang C, Ran J, Yang Q, . Effects of enamel matrix derivative on remineralization of initial enamel carious lesions in vitro. Archives of Oral Biology, 2013, 58(4): 362–369
[18]
Ran J M, Ieong C C, Xiang C Y, . In vitro inhibition of bovine enamel demineralization by enamel matrix derivative. Scanning,
CrossRef Google scholar
[19]
Moradian-Oldak J. Amelogenins: assembly, processing and control of crystal morphology. Matrix Biology, 2001, 20(5-6): 293–305
[20]
Du C, Falini G, Fermani S, . Supramolecular assembly of amelogenin nanospheres into birefringent microribbons. Science, 2005, 307(5714): 1450–1454
[21]
Fincham A G, Moradian-Oldak J, Diekwisch T G, . Evidence for amelogenin “nanospheres” as functional components of secretory-stage enamel matrix. Journal of Structural Biology, 1995, 115(1): 50–59
[22]
Moradian-Oldak J. Protein-mediated enamel mineralization. Frontiers in Bioscience, 2012, 17(7): 1996–2023
[23]
Fan Y, Sun Z, Moradian-Oldak J. Controlled remineralization of enamel in the presence of amelogenin and fluoride. Biomaterials, 2009, 30(4): 478–483
[24]
Fan Y, Nelson J R, Alvarez J R, . Amelogenin-assisted ex vivo remineralization of human enamel: Effects of supersaturation degree and fluoride concentration. Acta Biomaterialia, 2011, 7(5): 2293–2302
[25]
Porter S M. Seawater chemistry and early carbonate biomineralization. Science, 2007, 316(5829): 1302
[26]
Paine M L, Snead M L. Tooth developmental biology: disruptions to enamel-matrix assembly and its impact on biomineralization. Orthodontics & Craniofacial Research, 2005, 8(4): 239–251
[27]
Ieong C C, Zhou X D, Li J Y, . Possibilities and potential roles of the functional peptides based on enamel matrix proteins in promoting the remineralization of initial enamel caries. Medical Hypotheses, 2011, 76(3): 391–394
[28]
Cate J M T, Duijsters P P. Influence of fluoride in solution on tooth demineralization. I. Chemical data. Caries Research, 1983, 17(3): 193–199
[29]
White D J. Reactivity of fluoride dentifrices with artificial caries. I. Effects on early lesions: F uptake, surface hardening and remineralization. Caries Research, 1987, 21(2): 126–140
[30]
Snead M L, Zhu D H, Lei Y, . A simplified genetic design for mammalian enamel. Biomaterials, 2011, 32(12): 3151–3157
[31]
Lacruz R S, Smith C E, Bringas P Jr, . Identification of novel candidate genes involved in mineralization of dental enamel by genome-wide transcript profiling. Journal of Cellular Physiology, 2012, 227(5): 2264–2275
[32]
Aoba T, Tanabe T, Moreno E C. Proteins in the enamel fluid of immature porcine teeth. Journal of Dental Research, 1987, 66(12): 1721–1726
[33]
Iijima M, Moradian-Oldak J. Control of apatite crystal growth in a fluoride containing amelogenin-rich matrix. Biomaterials, 2005, 26(13): 1595–1603
[34]
Yamakoshi Y, Hu J C C, Ryu O H, . A comprehensive strategy for purifying pig enamel proteins in biomineralization: formation, diversity, evolution and application. In: Proceedings of the 8th International Symposium on Biomineralization. Hadano: Tokai University Press, 2003, 326-332
[35]
Luo J J, Ning T Y, Cao Y, . Biomimic enamel remineralization by hybridization calcium- and phosphate-loaded liposomes with amelogenin-inspired peptide. Key Engineering Materials, 2012, 512-515: 1727–1730
[36]
Roy M D, Stanley S K, Amis E J, . Identification of a highly specific hydroxyapatite-binding peptide using phage display. Advanced Materials, 2008, 20(10): 1830–1836
[37]
Ravindranath H H, Chen L S, Zeichner-David M, . Interaction between the enamel matrix proteins amelogenin and ameloblastin. Biochemical and Biophysical Research Communications, 2004, 323(3): 1075–1083
[38]
Beniash E, Simmer J P, Margolis H C. The effect of recombinant mouse amelogenins on the formation and organization of hydroxyapatite crystals in vitro. Journal of Structural Biology, 2005, 149(2): 182–190
[39]
Paine M L, Wang H J, Luo W, . A transgenic animal model resembling amelogenesis imperfecta related to ameloblastin overexpression. The Journal of Biological Chemistry, 2003, 278(21): 19447–19452
[40]
Habelitz S, DenBesten P K, Marshall S J, . Self-assembly and effect on crystal growth of the leucine-rich amelogenin peptide. European Journal of Oral Sciences, 2006, 114(Suppl 1): 315–319
[41]
Moradian-Oldak J, Tan J, Fincham A G. Interaction of amelogenin with hydroxyapatite crystals: an adherence effect through amelogenin molecular self-association. Biopolymers, 1998, 46(4): 225–238
[42]
Wallwork M L, Kirkham J, Chen H, . Binding of dentin noncollagenous matrix proteins to biological mineral crystals: an atomic force microscopy study. Calcified Tissue International, 2002, 71(3): 249–256
[43]
Müller H, Zentel R, Janshoff A, . Control of CaCO3 crystallization by demixing of monolayers. Langmuir, 2006, 22(26): 11034–11040
[44]
Bagheri H G, Sadr A, Espigares J, . Leucine rich amelogenin peptide improves the remineralization of enamel lesions. Dental Materials, 2014, 30(suppl): e172-e173
[45]
Tartaix P H, Doulaverakis M, George A, . In vitro effects of dentin matrix protein-1 on hydroxyapatite formation provide insights into in vivo functions. The Journal of Biological Chemistry, 2004, 279(18): 18115–18120
[46]
He G, Dahl T, Veis A, . Nucleation of apatite crystals in vitro by self-assembled dentin matrix protein 1. Nature Materials, 2003, 2(8): 552–558
[47]
Aggeli A, Bell M, Boden N, . Self-assembling peptide polyelectrolyte beta-sheet complexes form nematic hydrogels. Angewandte Chemie International Edition in English, 2003, 42(45): 5603–5606
[48]
Kirkham J, Firth A, Vernals D, . Self-assembling peptide scaffolds promote enamel remineralization. Journal of Dental Research, 2007, 86(5): 426–430
[49]
Hartgerink J D, Beniash E, Stupp S I. Self-assembly and mineralization of peptide-amphiphile nanofibers. Science, 2001, 294(5547): 1684–1688

Disclosure of potential conflicts of interests

We declare that we do not have any commercial or associative interest that represents a conflict of interest in connection with this work published.
This work was supported by the National Natural Science Foundation of China (Grant Nos. 81000431 and 81271128) and the New Century Excellent Talents University Support Programme. In addition we thank the Crest Research Laboratory of Procter & Gamble Technology (Beijing) Co. Ltd. for help with the transverse microradiography analyses.

RIGHTS & PERMISSIONS

2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
AI Summary AI Mindmap
PDF(5079 KB)

Accesses

Citations

Detail

Sections
Recommended

/