Surface modification of apatite--wollastonite glass ceramic by synthetic coupling agent

Abstract

In this study, lysine was introduced into the surface of apatite--wollastonite glass ceramic (AW-GC) to improve its cytocompatibility by two steps reaction procedure. Firstly, lysine connected to N-β-(aminoethyl)-γ-aminopropyl trimethoxy silane (A-1120) by covalent binding of amide group. Secondly, the lysine-functionalized A-1120 was deposited on the surface of AW-GC through a silanization reaction involving a covalent attachment. FTIR spectra indicated that lysine had been immobilized onto the surface of AW-GC successfully. Bioactivity of the surface modified AW-GC was investigated by simulated body fluid (SBF), and the in vitro cytocompatibility was evaluated by co-culturing with human osteosarcoma cell MG63. The results showed that the process of hydroxyapatite layer formed on the modified material was similar to AW-GC while the mode of hydroxyapatite deposition was changed. The growth of MG63 cells showed that modifying the AW-GC surface with lysine enhances the cell adhesion and proliferation.

Keywords

surface modification / apatite--wollastonite glass ceramic (AW-GC) / lysine / silane coupling agent / cytocompatibility

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Acknowledgements

This study was supported by the Specialized Research Fund for the Doctoral Program of Higher Education, China (Grant No. 20060610024).

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg