Bone regeneration using coculture of mesenchymal stem cells and angiogenic cells

Jin-Ling MA, Jeroen J. J. P. van den BEUCKEN, Ju-Li PAN, Fu-Zhai CUI, Su CHEN

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PDF(120 KB)
Front. Mater. Sci. ›› 2014, Vol. 8 ›› Issue (1) : 32-38. DOI: 10.1007/s11706-014-0236-x
MINI-REVIEW

Bone regeneration using coculture of mesenchymal stem cells and angiogenic cells

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Abstract

Cellular strategies remain a crucial component in bone tissue engineering (BTE). So far, the outcome of cell-based strategies from initial clinical trials is far behind compared to animal studies, which is suggested to be related to insufficient nutrient and oxygen supply inside the tissue-engineered constructs. Cocultures, by introducing angiogenic cells into osteogenic cell cultures, might provide a solution for improving vascularization and hence increasing bone formation for cell-based constructs. So far, pre-clinical studies demonstrated that cocultures enhance vascularization and bone formation compared to monocultures. However, there has been no report on the application of cocultures in clinics. Therefore, this mini-review aims to provide an overview regarding (i) critical parameters in cocultures and the outcomes of cocultures compared to monocultures in the currently available pre-clinical studies using human mesenchymal stem cells implanted in orthotopic animal models; and (ii) the usage of monocultures in clinical application in BTE.

Keywords

mesenchymal stem cell (MSC) / endothelial cell (EC) / coculture / vasculari-zation / tissue regeneration

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Jin-Ling MA, Jeroen J. J. P. van den BEUCKEN, Ju-Li PAN, Fu-Zhai CUI, Su CHEN. Bone regeneration using coculture of mesenchymal stem cells and angiogenic cells. Front. Mater. Sci., 2014, 8(1): 32‒38 https://doi.org/10.1007/s11706-014-0236-x

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2014 Higher Education Press and Springer-Verlag Berlin Heidelberg
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