Effect of Mg 2+ concentration on biocompatibility of pure magnesium

Abstract

The aim of the present study is to find the correlation between the Mg²⁺ concentration degraded from pure magnesium material and the biocompatibility of the material. Hemolysis ratio (HR) of the extracts of pure magnesium with different Mg²⁺ concentration were measured according to ISO 10993.4 standard. The cytotoxicity tests were carried out by both indirect contact with fibroblast L929 and preosteoblasts MC3T3-E1, and MTT tests were used. Cytotoxicity of the pure magnesium with and without surface modification was further evaluated by direct contact method. Samples were cultured with Osteoblast MC3T3-E1 and the effects of the material on viability and activity of cells were discussed. The results showed that the hemolysis rate and cytotoxicity of the modified Mg could meet the requirement for biomaterials. In our test, the hemolysis rate of the extracts was qualified when the concentration of Mg²⁺£ 42 mg/L; the extracts with 202 mg/L Mg²⁺ met the cytotoxicity requirement, and the extracts with 156 mg/L Mg²⁺ promoted cell proliferation.€Therefore, the€biocompatibili-ty of magnesium-based materials can be improved by suitable surface modification.

Keywords

magnesium / biomaterials / surface modification / biocompatibility