Proliferation of osteoblast cells on nanotubes

Abstract

Carbon nanotubes (CNT) have a unique structure and feature. In the present study, cell proliferation was performed on the scaffolds of single-walled CNTs (SWCNT), multiwalled CNTs (MWCNT), and on graphite, one of the representative isomorphs of pure carbon, for the sake of comparison. Scanning electron microscopy observation of the growth of osteoblast-like cells (Saos2) cultured on CNTs showed the morphology fully developed for the whole direction, which is different from that extended to one direction on the usual scaffold. Numerous filopodia were grown from cell edge, extended far long and combined with the CNT meshwork. CNTs showed the affinity for collagen and proteins. Proliferated cell numbers are largest on SWCNTs, followed by MWCNTs, and are very low on graphite. This is in good agreement with the sequence in the results of the adsorbed amount of proteins and expression of alkaline phosphatase activity for these scaffolds. The adsorption of proteins would be one of the most influential factors to make a contrast difference in cell attachment and proliferation between graphite and CNTs, both of which are isomorphs of carbon and composed of similar graphene sheet crystal structure. In addition, the nanosize meshwork structure with large porosity is another property responsible for the excellent cell adhesion and growth on CNTs. CNTs could be the favorable materials for biomedical applications.

Keywords

carbon nanotube / scaffold / osteoblast / regeneration / nanomaterial

References

[1] Ushiro M, Uno K, Fujikawa T, . X-ray absorption fine structure (XAFS) analyses of Ni species trapped in graphene sheet of carbon nanofibers. Physical Review B: Condensed Matter, 2006, 73: <patent>144103</patent>/1-11

[2] Yokoyama A, Sato Y, Nodasaka Y, . Biological behavior of hat-stacked carbon nanofibers in the subcutaneous tissue in rats. Nano Letters, 2005, 5: 157-161

[3] Sato Y, Shibata H. Kataoka S, . Strict preparation and evaluation of water-soluble hat-stacked carbon nanofibers for biomaedical application and their high biocompatibility: Influence of nanofiber-surface functional groups on cytotoxicity. Molecular Biosystems, 2005, 1: 142-145

[4] Watari F, Tamura K, Yokoyama A, . Biochemical and pathological responses of cells and tissue to micro- and nanoparticles from titanium and other materials. In: Bauerlein E. Handbook of Biomineralization. Weinheim: Wiley-VCH, 2007, 3: 127-144

[5] Kumazawa R, Watari F, Takashi N, . Effects of Ti ions and particles on cellular function and morphology of neutrophils. Biomaterials, 2002, 23: 3757-3764

[6] Tamura K, Takashi N, Kumazawa R, . Effects of particle size on cell function and morphology in titanium and nickel. Materials Transactions, 2002, 43: 3052-3057

[7] Watari F, Inoue M, Akasaka T, . Comparison of morphology and behavior of carbon nanotubes and asbestos. Proceedings of the 6thAsian BioCeramics Symposium, 2006, 142-145

[8] Kiura K, Sato Y, Yasuda M, . Activation of human monocytes and mouse splenocytes by single-walled carbon nanotubes. Journal of Biomedical Nanotechnology, 2005, 1: 359-364

[9] Sato Y, Yokoyama A, Shibata K, . Influence of length on cytotoxicity of multi-walled carbon nanotubes against human acute monocytic leukemia cell line THP-1 in vitro and subcutaneous tissue of rats in vivo. Molecular BioSystems, 2005, 1: 176-182

[10] Yokoyama A, Gelinsky M, Kawasaki T, . Biomimetic porous scaffolds with high elasticity made from mineralized collagen—an animal study. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2005, 75B: 464-472

[11] Gelinsky M, Bernhardt A, Eckert M, . Biomaterials based on mineralised collagen an artificial extracellular bone matrix. In: Watanabe M, Okuno O. Interface Oral Health Science. Japan. Springer, 2007, 323-328

[12] Li X M, van Blitterswijk C A, Feng Q L, . The effect of calcium phosphate microstructure on bone-related cells in vitro. Biomaterials, 2008, 29: 3306-3316

[13] Liao S, Wang W, Uo M, . A three-layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane for guided tissue regeneration. Biomaterials, 2005, 26: 7564-7571

[14] Liao S, Watari F, Zhu Y, . The degradation of the three layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane in vitro. Dental Materials, 2007, 23: 1120-1128

[15] Liao S, Xu G, Wang W, . Self-assembly of nano-hydroxyapatite on multi-walled carbon nanotubes. Acta Biomaterialia, 2007, 3: 669-675

[16] Wang W, Watari F, Omori M, . Mechanical properties and biological behavior of carbon nanotube/polycarbosilane composites for implant materials. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2007, 82: 223-230

[17] Rosca I D, Watari F, Uo M, . Oxidation of multiwalled carbon nanotubes by nitric acid. Carbon, 2005, 43: 3124-3131

[18] Aoki N, Akasaka T, Watari F, . Carbon nanotubes as scaffolds for cell and effect on cellular functions. Dental Materials Journal, 2007, 26: 178-185

[19] Aoki N, Yokoyama A, Nodasaka Y, . Cell culture on a carbon nanotube scaffold. Journal of Biomedical Nanotechnology, 2005, 1: 402-405

[20] Aoki N, Yokoyama A, Nodasaka Y, . Carbon nanotubes deposited on titanium implant for osteoblast attachment. Journal of Bionanoscience, 2007, 1: 14-16

[21] Akasaka T, Watari F, Sato Y, . Apatite formation on carbon nanotubes. Materials Science and Engineering C, 2005, 26: 675-678

[22] Akasaka T, Watari F. Nano-architechture on carbon nanotube surface by biomimetic coating. Chemistry Letters, 2005, 34: 826-827

[23] Li X M, Gao H, Uo M, . Effect of carbon nanotubes on cellular functions in vitro. Journal of Biomedical Materials Research Part A, 2008,DOI: 10.1002/jbm.a.32203

[24] Li X M, Gao H, Uo M, . Maturation of osteoblast-like Saos2 induced by carbon nanotubes. Biomedical Materials, 2009,DOI: 10.1008/17486041/4/1/015005

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Higher Education Press and Springer-Verlag Berlin Heidelberg