Release of lysozyme from electrospun PVA/lysozyme-gelatin scaffolds

Abstract

This article describes an electrospinning process in fabricating ultra fine fibers with core-shell structure. A biodegradable polymer, poly(vinyl alcohol) (PVA), was used as the shell; lysozyme was a kind of antioxidant; and gelatin were used as the core. Morphology and microstructure of the ultra fine fibers were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) analysis. As a comparison, composite nanofiber PVA/lysozyme-gelatin blend was prepared by a normal electrospinning process. In vitro drug release behaviors of the nanofibrous membranes were determined in phosphate-buffered saline (PBS) solution. It was found that core-shell nanofibers PVA/lysozyme-gelatin obviously exhibit higher initial release rates compared to that of PVA/lysozyme-gelatin blend nanofibers. The current method may find wide application in controlled release of bioactive proteins and tissue engineering.

References

1. Yoshimoto H, Shin Y M, Terai H, et al.. A biodegradable nanofiber scaffold by electrospinningand its potential for bone tissue engineering. Biomaterials, 2003, 24: 2077–2082. doi:10.1016/S0142‐9612(02)00635‐X
2. Shin M, Ishii O, Sueda T, et al.. Contractile cardiac grafts using a novel nanofibrousmesh. Biomaterials, 2004, 25: 3717–3723. doi:10.1016/j.biomaterials.2003.10.055
3. Kenawy El-R, Bowlin G L, Mansfield K, et al.. Release of tetracycline hydrochloride from electrospunpoly(ethylene-co-vinylacetate), poly(lactic acid), and a blend. Journal of Control Release, 2002, 81: 57–64. doi:10.1016/S0168‐3659(02)00041‐X
4. Luu Y K, Kim K, Hsiao B S, et al.. Development of a nanostructured DNA deliveryscaffold via electrospinning of PLGA and PLA–PEG block copolymers. Journal of Control Release, 2003, 89: 341–353. doi:10.1016/S0168‐3659(03)00097‐X
5. Zong X H, Ran S F, Kim K, et al.. Structure and morphology changes during in vitrodegradation of electrospun poly(glycolide-co-lactide) nanofiber membrane. Biomacromolecules, 2003, 4: 416–423. doi:10.1021/bm025717o
6. Zeng J, Xu X Y, Chen X S, et al.. Biodegradable electrospun fibers for drug delivery. Journal of Control Release, 2003, 92: 227–231. doi:10.1016/S0168‐3659(03)00372‐9
7. Katti D S, Robinson K W, Ko F K, et al.. Bioresorbable nanofiber-based systems for woundhealing and drug delivery: Optimization of fabrication parameters. Journal of Biomedical Materials Research Part B:Applied Biomaterials, 2004, 70B: 286–296. doi:10.1002/jbm.b.30041
8. Kim K, Luu Y K, Chang C, et al.. Incorporation and controlled release of a hydrophilicantibiotic using poly(lactide-co-glycolide)-based electrospun nanofibrousscaffolds. Journal of Control Release, 2004, 9: 47–56. doi:10.1016/j.jconrel.2004.04.009
9. Sanders E H, Kloefkorn R, Bowlin G L, et al.. Two-phase electrospinning from a single electrifiedjet: microencapsulation of aqueous reservoirs in poly(ethylene-co-vinylacetate) fibers. Macromolecules, 2003, 36: 3803–3805. doi:10.1021/ma021771l
10. Sun Z C, Zussman E, Yarin A L, et al.. Compound core-shell polymer nanofibers by co-electrospinning. Advanced Materials, 2003, 15: 1929–1932. doi:10.1002/adma.200305136
11. Zhang Y Z, Huang Z M, Xu X J, et al.. Preparation of core-shell structured PCL-r-Gelatinbi-component nanofibers by coaxial electrospinning. Chemical of Materials, 2004, 16: 3406–3409. doi:10.1021/cm049580f
12. Koombhongse S, Liu W, Reneker D H . Flat polymer ribbons and other shapes by electrospinning. Journal of Polymer Science, Part B: Polymer Physics, 2001, 39: 2598–2606. doi:10.1002/polb.10015
13. Yu J H, Fridrikh S V, Rutledge G C. Production of submicrometerdiameter fibers by Two-Fluid Electrospinning. Advanced Materials, 2004, 16: 1562–1566. doi:10.1002/adma.200306644
14. Li D, Xia Y N . Direct fabrication of compositeand ceramic hollow nanofibers by electrospinning. Nano Letters, 2004, 4: 933–938. doi:10.1021/nl049590f
15. Zhang Y Z, Wang X, Feng Y, et al.. Coaxial electrospinning of (fluorescein isothiocyanate-conjugatedbovine serum albumin)-encapsulated poly(-caprolactone) nanofibersfor sustained release. Biomacromolecules, 2006, 7: 1049–1057. doi:10.1021/bm050743i