Fast deposition of hydroxyapatite coating on titanium to modify cell affinity of corneal fibroblast in vitro

Abstract

By two step acid-alkali pretreatment and immersing into supersaturated calcification solution, hydroxyapatite (HA) coating was deposited on titanium (Ti) discs. The composition, surface morphology and cross-section of the coating were analyzed by X-ray diffraction (XRD) and scanning electron microscopy (SEM). Fibroblasts of rabbit cornea were seeded on HA coated Ti disc, pure Ti disc and glass. Cell adhesion, proliferation and morphology were detected at 24, 48 and 72 h, respectively. It is shown for the first time that HA coating can significantly enhance the adhesion and proliferation of rabbit corneal fibroblast in comparison with that of pure Ti.

Keywords

hydroxyapatite (HA) coating, titanium (Ti), cornea, fibroblast