Nitrogen-doped graphene approach to enhance the performance of a membraneless enzymatic biofuel cell

Abstract

Heteroatom-doping of pristine graphene is an effective route for tailoring new characteristics in terms of catalytic performance which opens up potentials for new applications in energy conversion and storage devices. Nitrogen-doped graphene (N-graphene), for instance, has shown excellent performance in many electrochemical systems involving oxygen reduction reaction (ORR), and more recently glucose oxidation. Owing to the excellent {\mathrm{H}}_2{\mathrm{O}}_2 sensitivity of N-graphene, the development of highly sensitive and fast-response enzymatic biosensors is made possible. However, a question that needs to be addressed is whether or not improving the anodic response to glucose detection leads to a higher overall performance of enzymatic biofuel cell (eBFC). Thus, here we first synthesized N-graphene via a catalyst-free single-step thermal process, and made use of it as the biocatalyst support in a membraneless eBFC to identify its role in altering the performance characteristics. Our findings demonstrate that the electron accepting nitrogen sites in the graphene structure enhances the electron transfer efficiency between the mediator (redox polymer), redox active site of the enzymes, and electrode surface. Moreover, the best performance in terms of power output and current density of eBFCs was observed when the bioanode was modified with highly doped N-graphene.

Key words： enzymatic fuel cell; nitrogen-doped graphene; reduced graphene oxide; catalyst-free synthesis

Acknowledgments

We highly thank Dr. Shelley D. Minteer and Dr. David P. Hickey (University of Utah) and Dr. Ross D. Milton (Stanford University) for kindly providing us with An-MWCNT and for their guidelines and support. This work also made use of instruments in the Electron Microscopy Service (EMS), at the Research Resources Center, University of Illinois at Chicago.

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