UPF3B promotes proliferation of malignant tumor cells in upper gastrointestinal tract

Abstract

Objective To investigate the effect of UPF3B on the proliferation of malignant cells inUpper gastrointestinal malignant tract. Methods Interference and overexpression efficiency of UPF3B in gastric and esophageal cancer cells verified by qRT-PCR and Western blot; the proliferation ability of cells was detected by CCK-8, EdU, and plate colony formation assay. Results siRNA knockdown of UPF3B expression levels in gastric and esophageal cancer cells significantly decreased the mRNA and protein expression levels of UPF3B, and was statistically significant (P<0.001), and CCK8, EdU and plate colony formation assay showed that knockdown of UPF3B inhibited the proliferation of gastric and esophageal cancer cells; transfection of UPF3B overexpression plasmid in gastric and esophageal cancer cells up-regulated the expression levels of UPF3B, and the mRNA and protein expression levels of UPF3B were significantly increased, and was statistically significant (P<0.001), and the results of CCK8, EdU and plate colony formation assay showed that overexpression of UPF3B promoted the proliferation of gastric and esophageal cancer cells. Conclusion UPF3B promotes the proliferation of malignant tumor cells in upper gastrointestinal tract.

Keywords

Upper gastrointestinal malignant tract / Esophageal cancer / Gastric cancer / UPF3B / proliferation