Enhancement of extracellular Cr(VI) reduction for anammox recovery using hydrazine: performance, pathways, and mechanism

Highlights

● N₂H₄ addition enhanced and recovered anammox performance under Cr(VI) stress.

● N₂H₄ accelerated electron transfer of Cr(VI) reduction for detoxification.

● N₂H₄ enhanced anammox metabolism for activity recovery from Cr(VI) inhibition.

● Extracellular Cr(VI) reduction to less toxic Cr(III) was the dominant mechanism.

Abstract

The hexavalent chromium (Cr(VI)) would frequently impose inhibition to anaerobic ammonium oxidation (anammox) process, hindering the efficiency of nitrogen removal in wastewater treatment. Hydrazine (N₂H₄), which is an intermediate product of anammox, participates in intracellular metabolism and extracellular Cr(VI) reduction. However, the roles of N₂H₄-induced intracellular metabolism and extracellular reduction in nitrogen removal under Cr(VI) stress remain unclear. The addition of 3.67 mg/L of N₂H₄ increased the anammox activity by 17%. As an intermediate, N₂H₄ enhanced anammox metabolism by increasing the heme c content and electron transfer system activity. As a reductant, N₂H₄ accelerated the reduction of c-Cyts-mediated extracellular Cr(VI) to the less toxic Cr(III). Extracellular Cr(III) accounts for 74% of the total Cr in a Cr(VI)-stressed anammox consortia. These findings highlight that N₂H₄-induced extracellular Cr(VI) reduction is the dominant mechanism for the survival of anammox consortia. We also found that N₂H₄ increased the production of extracellular polymeric substances to sequester excessive Cr(VI) and produced Cr(III). Taken together, the study findings suggest a potential strategy for enhancing nitrogen removal from ammonium-rich wastewater contaminated with Cr(VI).

Graphical abstract

Keywords

Extracellular Cr(VI) reduction / Electron transfer / Anammox / Hydrazine / Cr(VI) inhibition

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Acknowledgements

This work is supported by the National Natural Science Foundations of China (No. 52000182, U21A20294, 51878662), the Natural Science Foundation of Hunan Province (No. 2020JJ4725) and the Technological Innovation Guidance Program of Jiangxi Province (No. 20203BDH80W017). Dr. Tang C J is supported by the Young Scholar Program of Ministry of Education of China.

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11783-023-1715-z and is accessible for authorized users.

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