Enhanced methane production during long-term UASB operation at high organic loads as enabled by the immobilized Fungi

Highlights

• Fungi enable the constant UASB operation even at OLR of 25.0 kg/(m³×d).

• The COD removal of 85.9% and methane production of 5.6 m³/(m³×d) are achieved.

• Fungi inhibit VFAs accumulation and favor EPS generation and sludge granulation.

• Fungi enrich methanogenic archaea and promote methanogenic pathways.

Abstract

Anaerobic digestion is widely applied in organic wastewater treatment coupled with bioenergy production, and how to stabilize its work at the high organic loading rate (OLR) remains a challenge. Herein, we proposed a new strategy to address this issue via involving the synergetic role of the Aspergillus sydowii 8L-9-F02 immobilized beads (AEBs). A long-term (210-day) continuous-mode operation indicated that the upflow anaerobic sludge bed (UASB) reactor (R1, with AEBs added) could achieve the OLR as high as 25.0 kg/(m³×d), whereas the control reactor (R0, with AEBs free) could only tolerate the maximum OLR of 13.3 kg/(m³×d). Remarkably, much higher COD removal (85.9% vs 23.9%) and methane production (5.4 m³/(m³×d) vs 2.2 m³/(m³×d)) were achieved in R1 than R0 at the OLR of 25.0 kg/(m³×d). Such favorable effect results from the facts that fungi inhibit VFAs accumulation, favor the pH stabilization, promote the generation of more extracellular polymeric substance, and enhance the sludge granulation and settleability. Moreover, fungi may enhance the secretion of acetyl-coenzyme A, a key compound in converting organic matters to CO₂. In addition, fungi are favorable to enrich methanogenic archaea even at high OLR, improving the activity of acetate kinase and coenzyme F₄₂₀ for more efficient methanogenic pathway. This work may shed new light on how to achieve higher OLR and methane production in anaerobic digestion of wastewater.

Graphical abstract

Keywords

Anaerobic digestion / Fungi / Methane production / High OLR / Microbial community

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Acknowledgements

This study was financially supported from the Natural Science Foundation of China (Grant No. 21925602 and 51761165011).

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11783-021-1505-4 and is accessible for authorized users.

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