Metagenomic analysis on resistance genes in water and microplastics from a mariculture system

Highlights

• Total 174 subtypes of ARGs were detected by metagenomic analysis.

• Chloramphenicol resistance genes were the dominant ARGs in water and microplastics.

• The abundances of MRGs were much higher than those of ARGs.

• Proteobacteria, Bacteroidetes, and Actinobacteria were the dominant phylum.

• Microplastics in mariculture system could enrich most of MRGs and some ARGs.

Abstract

Microplastics existing widely in different matrices have been regarded as a reservoir for emerging contaminants. Mariculture systems have been observed to host microplastics and antibiotic resistance genes (ARGs). However, more information on proliferation of ARGs and metal resistance genes (MRGs) in mariculture system at the presence of microplastics is needed. This study used metagenomic analysis to investigate the distribution of ARGs and MRGs in water and microplastics of a typical mariculture pond. Total 18 types including 174 subtypes of ARGs were detected with the total relative abundances of 1.22/1.25 copies per 16S rRNA copy for microplastics/water. Chloramphenicol resistance genes were the dominant ARGs with the abundance of 0.35/0.42 copies per 16S rRNA copy for microplastics/water. Intergron intI1 was dominant gene among 6 detected mobile genetic elements (MGEs) with the abundance of 75.46/68.70 copies per 16S rRNA copy for water/microplastics. Total 9 types including 46 subtypes of MRGs were detected with total abundance of 5.02 × 10²/6.39 × 10² copies per 16S rRNA copy for water/ microplastics while genes resistant to copper and iron served as the dominant MRGs. Proteobacteria, Bacteroidetes, and Actinobacteria accounted for 84.2%/89.5% of total microbial community. ARGs with relatively high abundance were significantly positively related to major genera, MGEs, and MRGs. Microplastics in mariculture system could enrich most of MRGs and some ARGs to serve as potential reservoir for these pollutants. The findings of this study will provide important information on resistance gene pollution at presence of microplastics in the mariculture system for further proposing suitable strategy of environmental management.

Graphical abstract

Keywords

Antibiotic resistance genes / Metal resistance genes / Metagenomic analysis / Microplastics / Mariculture

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Grant No. 41877131), Taishan Scholars Program of Shandong Province (No. tsqn201812116), Science and Technology Service Network Initiative of the Chinese Academy of Sciences (KFJ-STS-QYZX-114), Two-Hundred Talents Plan of Yantai (No. Y739011021), and Wanhua Chemical Group Co. Ltd.

Electronic Supplementary Material

Supplementary material is available in the online version of this article at https://doi.org/10.1007/s11783-021-1438-y and is accessible for authorized users.

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