Isolation and application of predatory Bdellovibrio-and-like organisms for municipal waste sludge biolysis and dewaterability enhancement

Abstract

Indigenous predatory BALO strains were successfully isolated from activated sludge.

Sludge SRF and CST were significantly reduced by BALOs induced biolysis process.

The increase of BALO input dosage promoted the sludge biolysis efficiency.

Sludge biolysis disintegrated flocs and lysed cells for internal water release.

The optimal sludge biolysis time was 24 h and no pH adjustment was needed.

Bdellovibrio-and-like organisms (BALOs) are a group of ubiquitous and obligate predatory bacteria and commonly used as biocontrol agents. In this study, an efficient, environmental-friendly, and convenient BALOs encouraged municipal waste sludge biolysis pretreatment technique was developed and investigated for dewaterability enhancement of excess waste sludge. The indigenous predatory BALOs were successfully isolated from the sludge for biolysis treatment. Without any chemical addition or pH adjustment, the sludge specific resistance (SRF) and capillary suction time (CST) were significantly reduced by as high as 53.4% and 23.8%, respectively within 24 h’s treatment, which would further be lowered with the increase of BALOs input dosage. However, the continuous extension of reaction time would worsen the sludge dewaterability. The decreases of SRF and CST accompanied with the increases of sludge disintegration degree and soluble chemical oxygen demand, nitrogen, and phosphorus concentrations all emphasized the contributions of BALOs’ predation activities to sludge disturbance, cell lysis, and consequently the release of sludge intracellular water to finally effectively improve the sludge dewaterability and disposal efficiency.

Graphical abstract

Keywords

Bdellovibrio-and-like organisms (BALOs) / Biolysis / Activated sludge / Dewaterability / Predation

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant No. 51208092) and Technology Foundation for Selected Overseas Chinese Scholar, Ministry of Human Resources and Social Security of the Peoples’ Republic China (2014).

Funding

RIGHTS & PERMISSIONS

2017 Higher Education Press and Springer-Verlag Berlin Heidelberg