Effect of dilution rate on dynamic and steady-state biofilm characteristics during phenol biodegradation by immobilized Pseudomonas desmolyticum cells in a pulsed plate bioreactor

Veena Bangalore Rangappa, Vidya Shetty Kodialbail, Saidutta Malur Bharthaiyengar

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Front. Environ. Sci. Eng. ›› 2016, Vol. 10 ›› Issue (4) : 16. DOI: 10.1007/s11783-016-0863-9
RESEARCH ARTICLE

Effect of dilution rate on dynamic and steady-state biofilm characteristics during phenol biodegradation by immobilized Pseudomonas desmolyticum cells in a pulsed plate bioreactor

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Abstract

Continuous pulsed plate bioreactor (PPBR) was used for phenol biodegradation.

Pseudomonas desmolyticum cells immobilized on granular activated carbon was used.

Dynamic and steady state biofilm characteristics depend on dilution rate (DR).

Lower DR favour phenol degradation and uniform, thick biofilm formation.

Exo polymeric substance production in biofilm are favoured at lower dilution rates.

Pulsed plate bioreactor (PPBR) is a biofilm reactor which has been proven to be very efficient in phenol biodegradation. The present paper reports the studies on the effect of dilution rate on the physical, chemical and morphological characteristics of biofilms formed by the cells of Pseudomonas desmolyticum on granular activated carbon (GAC) in PPBR during biodegradation of phenol. The percentage degradation of phenol decreased from 99% to 73% with an increase in dilution rate from 0.33 h1 to 0.99 h1 showing that residence time in the reactor governs the phenol removal efficiency rather than the external mass transfer limitations. Lower dilution rates favor higher production of biomass, extracellular polymeric substances (EPS) as well as the protein, carbohydrate and humic substances content of EPS. Increase in dilution rate leads to decrease in biofilm thickness, biofilm dry density, and attached dry biomass, transforming the biofilm from dense, smooth compact structure to a rough and patchy structure. Thus, the performance of PPBR in terms of dynamic and steady-state biofilm characteristics associated with phenol biodegradation is a strong function of dilution rate. Operation of PPBR at lower dilution rates is recommended for continuous biologic treatment of wastewaters for phenol removal.

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Keywords

Biofilm / Exopolymeric substances / Phenol / Dilution rate / Pulsed plate bioreactor

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Veena Bangalore Rangappa, Vidya Shetty Kodialbail, Saidutta Malur Bharthaiyengar. Effect of dilution rate on dynamic and steady-state biofilm characteristics during phenol biodegradation by immobilized Pseudomonas desmolyticum cells in a pulsed plate bioreactor. Front. Environ. Sci. Eng., 2016, 10(4): 16 https://doi.org/10.1007/s11783-016-0863-9

References

[1]
Al-Khalid T, El-Naas M H. Aerobic biodegradation of phenols: a comprehensive review. Critical Reviews in Environmental Science and Technology, 2012, 42(16): 1631–1690
CrossRef Google scholar
[2]
Shetty K V, Kalifathulla I, Srinikethan G. Performance of pulsed plate bioreactor for biodegradation of phenol. Journal of Hazardous Materials, 2007, 140(1–2): 346–352
CrossRef Pubmed Google scholar
[3]
EPA. Priority Pollutant List (2014). http://www.epa.gov/sites/production/files/2015-09/documents/priority-pollutant-list-epa.pdf (date of access:<Date> February 1, 2016</Date>)
[4]
WHO. Guidelines for Drinking-water Quality (2011). 4th edition. http://apps.who.int/iris/bitstream/10665/44584/1/9789241548151_eng.pdf (Date of Access: <Date>February 2, 2016</Date>).
[5]
The Environment (Protection) Rules. http://cpcb.nic.in/GeneralStandards.pdf. (Date of Access: <Date>February 2, 2016</Date>)
[6]
Pishgar R, Najafpour G, Neya B N, Mousavi N, Bakhshi Z. Anaerobic biodegradation of phenol: Comparative study of free and immobilized growth. Iranica Journal of Energy and Environment, 2011, 2(4): 348–355
[7]
Dabhade M A, Saidutta M B, Murthy D V. Continuous phenol removal using Nocardia hydrocarbonoxydans in spouted bed contactor: shock load study. African Journal of Biotechnology, 2009, 8(4): 644–649
[8]
Wei Y, Yin X, Qi L, Wang H, Gong Y, Luo Y. Effects of carrier-attached biofillm on oxygen transfer effciency in a moving bed biofilm reactor. Frontiers in Environmental Science and Engineering, 2016, 10(3): 569–577
[9]
Vu B, Chen M, Crawford R J, Ivanova E P. Bacterial extracellular polysaccharides involved in biofilm formation. Molecules (Basel, Switzerland), 2009, 14(7): 2535–2554
CrossRef Pubmed Google scholar
[10]
Xue B, Hanchang S, Zhengfang Y, Qiujin S, Qing W, Zhongyou W. Degradation of bisphenol a by microorganisms immobilized on polyvinyl alcohol microspheres. Fronteirs in Environmental Science and Engineering, 2013, 7(6): 844–850
CrossRef Google scholar
[11]
Etzensperger M, Thoma S, Petrozzi S, Dunn I J. Phenol degradation in a three-phase biofilm fluidized sand bed reactor. Bioprocess Engineering, 1989, 4(4): 175–181
CrossRef Google scholar
[12]
Liu H, Fang H H. Extraction of extracellular polymeric substances (EPS) of sludges. Journal of Biotechnology, 2002, 95(3): 249–256
CrossRef Pubmed Google scholar
[13]
Kumar M A, Anandapandian K T K, Parthiban K. Production and characterization of exopolysaccharides (EPS) from biofilm forming marine bacterium. Brazilian Archives of Biology and Technology, 2011, 54(2): 259–265
CrossRef Google scholar
[14]
Andersson S, Dalhammar G, Land C J, Kuttuva Rajarao G. Characterization of extracellular polymeric substances from denitrifying organism Comamonas denitrificans. Applied Microbiology and Biotechnology, 2009, 82(3): 535–543
CrossRef Pubmed Google scholar
[15]
Marvasi M, Pieter T V, Lilliam C M. Exopolymeric substances (EPS) from Bacillus subtilis: polymers and genes encoding their synthesis. Federation of European Microbiology Societies, Microbiology Letters, 2010, 313: 1–9
[16]
Garny K, Horn H, Neu T R. Interaction between biofilm development, structure and detachment in rotating annular reactors. Bioprocess and Biosystems Engineering, 2008, 31(6): 619–629
CrossRef Pubmed Google scholar
[17]
Rittmann B E, Trinet F, Amar D, Chang H T. Measurement of the activity of a biofilm: Effects of surface loading and detachment on a three-phase, liquid-fluidized-bed reactor. Water Science and Technology, 1992, 26(3–4): 585–594
[18]
Furumai H, Rittmann B E. Evaluation of multiple-species biofilm and floc processes using a simplified aggregate model. Water Science and Technology, 1994, 29(10–11): 439–446
[19]
Wasche S, Horn H, Hempel D. Mass transfer phenomena in biofilm systems. Water Science and Technology, 2000, 41(4–5): 357–360
[20]
Vidya Shetty K, Ramanjaneyulu R, Srinikethan G. Biological phenol removal using immobilized cells in a pulsed plate bioreactor: effect of dilution rate and influent phenol concentration. Journal of Hazardous Materials, 2007b, 149(2): 452–459
CrossRef Pubmed Google scholar
[21]
Veena B R, Vidya S K, Saidutta M B. Shear Stress Effects on Production of Exopolymeric Substances and Biofilm Characteristics During Phenol Biodegradation by Immobilized Pseudomonas desmolyticum (NCIM2112) Cells in a Pulsed Plate Bioreactor. Preparative Biochemistry and Biotechnology, 2015http://www.tandfonline.com/doi/full/10.1080/10826068.2015.1045605
[22]
Lowry O H, Rosebrough N J, Farr A L, Randall R J. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 1951, 193(1): 265–275 PMID:14907713
[23]
Frølund B, Griebe T, Nielsen P H. Enzymatic activity in the activated-sludge floc matrix. Applied Microbiology and Biotechnology, 1995, 43(4): 755–761
CrossRef Pubmed Google scholar
[24]
Dubois M, Gilles K A, Hamilton J K, Rebers P T, Smith F. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 1956, 28(3): 350–356
CrossRef Google scholar
[25]
Schaedler S, Burkhardt C, Kappler A. Evaluation of electron microscopic sample preparation methods and imaging techniques for characterization of cell-mineral aggregates. Geomicrobiology Journal, 2008, 25(5): 228–239
CrossRef Google scholar
[26]
Tang W T, Fan L S. Steady state phenol degradation in a draft‐tube, gas‐liquid‐solid fluidized‐bed bioreactor. American Institute of Chemical Engineers journal, 1987,33: 239–249
[27]
APHA. Standard Methods for the Examination of Water and Wastewater. American Public Health Association. 19th Edition. Washington, D.C: American Public Health Association, 1995
[28]
Cortez S, Teixeira P, Oliveira R, Mota M. Rotating biological contactors: a review on main factors affecting performance. Reviews in Environmental Science and Biotechnology, 2008, 7(2): 155–172
CrossRef Google scholar
[29]
More T T, Yadav J S S, Yan S, Tyagi R D, Surampalli R Y. Extracellular polymeric substances of bacteria and their potential environmental applications. Journal of Environmental Management, 2014, 144: 1–25
CrossRef Pubmed Google scholar
[30]
Denhaus E, Meisen S, Telgheder U, Wingender J. Chemical and physical methods for characterisation of biofilms. Mikrochimica Acta, 2007, 158(1–2): 1–27
CrossRef Google scholar
[31]
Şeker S, Haluk B, Tanyolaç A. The effects of biofilm thickness on biofilm density and substrate consumption rate in a differential fluidizied bed biofilm reactor (DFBBR). Journal of Biotechnology, 1995, 41(1): 39–47
CrossRef Google scholar
[32]
Tanyolac A, Haluk B. Prediction of substrate consumption rate, average biofilm density and active thickness for a thin spherical biofilm at pseudo-steady state. Biochemical Engineering Journal, 1998, 2(3): 207–216
CrossRef Google scholar
[33]
Liu Y, Tay J H. Detachment forces and their influence on the structure and metabolic behaviour of biofilms. World Journal of Microbiology & Biotechnology, 2007, 17(2): 111–117
CrossRef Google scholar
[34]
Nicolella C, Di Felice R, Rovatti M. Biomass concentration in fluidised bed biological reactors. Water Research, 1997, 31(4): 936–940
CrossRef Google scholar
[35]
Rabah F K, Dahab M F. Biofilm and biomass characteristics in high-performance fluidized-bed biofilm reactors. Water Research, 2004, 38(19): 4262–4270
CrossRef Pubmed Google scholar
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