Characterization of the dissolved organic matter in sewage effluent of sequence batch reactor: the impact of carbon source

Jin GUO, Feng SHENG, Jianhua GUO, Xiong YANG, Mintao MA, Yongzhen PENG

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Front. Environ. Sci. Eng. All Journals
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Front. Environ. Sci. Eng. ›› 2012, Vol. 6 ›› Issue (2) : 280-287. DOI: 10.1007/s11783-011-0336-0
RESEARCH ARTICLE
RESEARCH ARTICLE

Characterization of the dissolved organic matter in sewage effluent of sequence batch reactor: the impact of carbon source

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Abstract

Dissolved organic matter (DOM) transformation in sequence batch reactor (SBR) fed with carbon sources of different biodegradability was investigated. During the biologic degradation process, the low molecular weight (MW) fraction (<1 kDa) gradually decreased, while the refractory compounds with higher aromaticity were aggregated. Size exclusion chromatography (SEC) and fluorescence of excitation emission matrices (EEM) demonstrated that more biopolymers (polysaccharides or proteins) and humic-like substances were presented in the extracellular polymeric substance (EPS) extracted from the SBR fed with sodium acetate or glucose, while the EPS from SBR fed with slowly biodegradable dissolved organic carbon (DOC) substrate-starch had relatively less biopolymers. Comparing the EfOM in sewage effluent of three SBRs, the effluent from SBR fed with starch is more aromatic. Organic carbon with MW>1 kDa as well as the hydrophobic fraction in DOM gradually increased with the carbon sources changing from sodium acetate to glucose and starch. The DOC fractionation and the EEM all demonstrated that EfOM from the effluent of the SBR fed with starch contained more fulvic acid-like substances comparing with the SBR fed with sodium acetate and glucose.

Keywords

effluent organic matter (EfOM) / extracellular polymeric substance (EPS) / molecular weight distribution (MWD) / hydrophobic / hydrophilic / excitation emission matrices (EEM)

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Jin GUO, Feng SHENG, Jianhua GUO, Xiong YANG, Mintao MA, Yongzhen PENG. Characterization of the dissolved organic matter in sewage effluent of sequence batch reactor: the impact of carbon source. Front Envir Sci Eng, 2012, 6(2): 280‒287 https://doi.org/10.1007/s11783-011-0336-0
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References

[1]
Shon H K, Vigneswaran S, Snyder S A. Effluent organic matter (EfOM) in wastewater: constituents, effects, and treatment. Critical Reviews in Environmental Science and Technology, 2006, 36(4): 327-374
CrossRef Google scholar
[2]
Jarusutthirak C, Amy G. Understanding soluble microbial products (SMP) as a component of effluent organic matter (EfOM). Water Research, 2007, 41(12): 2787-2793
CrossRef Pubmed Google scholar
[3]
Shin H S, Kang S T. Characteristics and fates of soluble microbial products in ceramic membrane bioreactor at various sludge retention times. Water Research, 2003, 37(1): 121-127
CrossRef Pubmed Google scholar
[4]
Al-Halbouni D, Traber J, Lyko S, Wintgens T, Melin T, Tacke D, Janot A, Dott W, Hollender J. Correlation of EPS content in activated sludge at different sludge retention times with membrane fouling phenomena. Water Research, 2008, 42(6-7): 1475-1488
CrossRef Pubmed Google scholar
[5]
Kimura K, Yamato N, Yamamura H, Watanabe Y. Membrane fouling in pilot-scale membrane bioreactors (MBRs) treating municipal wastewater. Environmental Science & Technology, 2005, 39(16): 6293-6299
CrossRef Pubmed Google scholar
[6]
Langenhoff A A M, Intrachandra N, Stuckey D C. Treatment of dilute soluble and colloidal wastewater using an anaerobic baffled reactor: influence of hydraulic retention time. Water Research, 2000, 34(4): 1307-1317
CrossRef Google scholar
[7]
Garnier C, Görner T, Lartiges B S, Abdelouhab S, de Donato P. Characterization of activated sludge exopolymers from various origins: a combined size-exclusion chromatography and infrared microscopy study. Water Research, 2005, 39(13): 3044-3054
CrossRef Pubmed Google scholar
[8]
Li X Y, Yang S F. Influence of loosely bound extracellular polymeric substances (EPS) on the flocculation, sedimentation and dewaterability of activated sludge. Water Research, 2007, 41(5): 1022-1030
CrossRef Pubmed Google scholar
[9]
Yang S F, Li X Y. Influences of extracellular polymeric substances (EPS) on the characteristics of activated sludge under non-steady-state conditions. Process Biochemistry, 2009, 44(1): 91-96
CrossRef Google scholar
[10]
Miqueleto A P, Dolosic C C, Pozzi E, Foresti E, Zaiat M. Influence of carbon sources and C/N ratio on EPS production in anaerobic sequencing batch biofilm reactors for wastewater treatment. Bioresource Technology, 2010, 101(4): 1324-1330
CrossRef Pubmed Google scholar
[11]
Huang G T, Jin G, Wu J H, Liu Y D. Effects of glucose and phenol on soluble microbial products (SMP) in sequencing batch reactor systems. International Biodeterioration & Biodegradation, 2008, 62(2): 104-108
CrossRef Google scholar
[12]
Magbanua B S Jr, Bowers A R. Characterization of soluble microbial products (SMP) derived from glucose and phenol in dual substrate activated sludge bioreactors. Biotechnology and Bioengineering, 2006, 93(5): 862-870
CrossRef Pubmed Google scholar
[13]
Thurman E M, Malcolm R L. Preparative isolation of aquatic humic substances. Environmental Science & Technology, 1981, 15(4): 463-466
CrossRef Google scholar
[14]
Imai A, Fukushima T, Matsushige K, Kim Y H, Choi K. Characterization of dissolved organic matter in effluents from wastewater treatment plants. Water Research, 2002, 36(4): 859-870
CrossRef Pubmed Google scholar
[15]
Liu H, Fang H H P. Extraction of extracellular polymeric substances (EPS) of sludges. Journal of Biotechnology, 2002, 95(3): 249-256
CrossRef Pubmed Google scholar
[16]
Park N, Kwon B, Kim S D, Cho J. Characterizations of the colloidal and microbial organic matters with respect to membrane foulants. Journal of Membrane Science, 2006, 275(1-2): 29-36
CrossRef Google scholar
[17]
Shon H K, Vigneswaran S, Aim R B, Ngo H H, Kim I S, Cho J. Influence of flocculation and adsorption as pretreatment on the fouling of ultrafiltration and nanofiltration membranes: application with biologically treated sewage effluent. Environmental Science & Technology, 2005, 39(10): 3864-3871
CrossRef Pubmed Google scholar
[18]
Esparza-Soto M, Fox P, Westerhoff P. Transformation of molecular weight distributions of dissolved organic carbon and UV-absorbing compounds at full-scale wastewater-treatment plants. Water Environment Research, 2006, 78(3): 253-262
CrossRef Pubmed Google scholar
[19]
Nam S N, Amy G. Differentiation of wastewater effluent organic matter (EfOM) from natural organic matter (NOM) using multiple analytical techniques. Water Science and Technology, 2008, 57(7): 1009-1015
CrossRef Pubmed Google scholar
[20]
Dong M M, Mezyk S P, Rosario-ortiz F L. Reactivity of effluent organic matter (EfOM) with hydroxyl radical as a function of molecular weight. Environmental Science & Technology, 2010, 44(15): 5714-5720
CrossRef Pubmed Google scholar
[21]
Coble P G, Green S A, Blough N V, Gagosian R B. Characterization of dissolved organic matter in the black sea by fluorescence spectroscopy. Nature, 1990, 348(6300): 432-435
CrossRef Google scholar
[22]
Sheng G P, Yu H Q. Characterization of extracellular polymeric substances of aerobic and anaerobic sludge using three-dimensional excitation and emission matrix fluorescence spectroscopy. Water Research, 2006, 40(6): 1233-1239
CrossRef Pubmed Google scholar
[23]
Mobed J J, Hemmingsen S L, Autry J L, McGown L B. Fluorescence characterization of IHSS humic substances: total luminescence spectra with absorbance correction. Environmental Science & Technology, 1996, 30(10): 3061-3065
CrossRef Google scholar
[24]
Martins A M P, Karahan O, van Loosdrecht M C. Effect of polymeric substrate on sludge settleability. Water Research, 2011, 45(1): 263-273
CrossRef Pubmed Google scholar

Acknowledgements

The authors wish to acknowledge Water Pollution Control Research Center of Beijing University of Technology for the sampling assistant, and sincerely thank the fund supported by the National Science and Technology Commission of China (Grant No. 50808003), and the Doctoral Start-up Funds offered by Beijing University of Technology (No. X0005013200801).
Nomenclature
effluent organic matterEfOM
dissolved organic carbonDOC
dissolved organic matterDOM
biologically Treated Sewage EffluentBTSE
extracellular polymeric substanceEPS
soluble microbial productsSMP
sequence batch reactorSBR
solid retention times SRT
food-microorganisms ratio F/M
hydraulic retention time HRT
sludge volume indexSVI
non-purgeable organic carbonNPOC
specific ultraviolet absorbanceSUVA
size exclusion chromatographySEC
excitation emission matricesEEM
molecular weight distributionMWD
hydrophobic acidsHOA
hydrophobic neutralsHON
hydrophobic basesHOB
hydrophilic acidsHIA
hydrophilic neutralsHIN
hydrophilic basesHIB

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