Cell carrier function of hollow-fiber membrane in rotating wall vessel bioreactor

Abstract

Large-scale expansion of the osteoblasts of a Sprague-Dawley (SD) rat was studied in a rotating wall hollow-fiber membrane bioreactor (RWHMB) by using hollow-fiber membrane as the carrier. For the sake of contrast, cells were also expanded in a T-flask using a hollow-fiber membrane as carrier and in a rotating wall vessel bioreactor (RWVB) using a microcarrier. During the culture period, the cells were sampled every 12 h, and after 5 days, the cells were harvested and evaluated with scanning electron microscopy (SEM), hematoxylin-eosin (HE) staining and alkaline phosphatase (ALP) staining. Moreover, von-Kossa staining and Alizarin Red S staining were carried out for mineralized nodules formation. The results show that in RWHMB, the cells present better morphology and vitality and secrete much more extracellular matrix. It is concluded that the RWHMB combines the advantages of the rotating wall vessel and hollow-fiber membrane bioreactors. The hydrodynamic stimulation within it accelerates the metabolism of the osteoblast and mass transfer, which is propitious to cell differentiation and proliferation.

References

1. Cabral J M S Ex vivo expansion of hematopoietic stem cells in bioreactorsBiotechnol Lett 2001 23(1010)741751
2. Wang D Liu W Han B Xu R The bioreactor:a powerful tool for large-scale culture of animal cellsCurr Pharm Biotechnol 2005 6(5)397403
3. Portner R Nagel-Heyer S Goepfert C Goepfert C Adamietz P Meenen N M Bioreactordesign for tissue engineeringJ Biosci Bioeng 2005 100(3)235245
4. Martin Y Vermette P Bioreactors for tissue massculture: design, characterization, and recent advancesBiomaterials 2005 26(35)74817503
5. Martin I Wendt D Heberer M The role of bioreactors in tissue engineeringTrends Biotechnol 2004 22(2)8086
6. Van wezel A L Growthof cell-strains and primary cells on microcarriers in homogeneouscultureNature 1967 2166465
7. Song K D Liu T Q Cui Z F Li X Q Ge D Sun X Y Ma X H Large scale expansion of rat's osteoblastsin rotating wall vessel (rwv) bioreactorChin J Biomed Eng 2005 24(2)134139(in Chinese)
8. Kallos M S Sen A Behie L A Large-scale expansion of mammalian neural stem cells: areviewMed Biol Eng Comput 2003 41(3)271282
9. Bosch T Schmidt B Samtleben W Gurland H J Biocompatibilityand clinical performance of a new modified cellulose membraneClin Nephrol 1986 26(Suppl 1)S2229
10. Zhang W D Li A M Ren Z Q Mass transfer characteristics of hollow fiber renewal liquidmembraneJ Chem Eng of Chinese Univ 2006 20(5)843846(in Chinese)
11. Wang Y J Chen F Wang Y Luo G S Dai Y Y Mass transfer and hydrodynamics characteristicsof phenol extraction using hollow fiber moduleJ Chem Eng of Chinese Univ 2002 16(5)484489(in Chinese)
12. Curcio E De Bartolo L Barbieri G Rende M, Giorno L, Morelli S, Drioli E. Diffusive and convectivetransport through hollow fiber membranes for liver cell culture.J Biotechnol 2005 117(3)309321
13. Planchamp C Ivancevic M K Pastor C M Vallée J P Pochon S Terrier F Mayer J M Reist M Hollow fiber bioreactor: new developmentfor the study of contrast agent transport into hepatocytes by magneticresonance imagingBiotechnol Bioeng 2004 85(6)656665
14. Song K D Liu T Q Cui Z F Li X Q Ge D Sun X Y Ma X H Three-dimensional fabrication of engineeredbone in rotating wall vessel bioreactorProg Biochem Biophys 2004 31(11)9961005(in Chinese)
15. Botchwey E A Pollack S R Levine E M Laurencin. C T Bone tissue engineering ina rotating bioreactor using a micro carrier matrix systemJ Biomed Mater Res 2001 55(2)242253
16. Saris D B Sanyal A An K N Fitzsimmons J S O'Driscoll S W Periosteum responds to dynamicfluid pressure by proliferating in vitroJ Orthop Res 1999 17668677