The role of CyclinD1 and estrogen receptor (ER) in the process of proliferation and metastasis of breast neoplasm and their relationship were studied. The expression levels of CyclinD1 and ER in the tissue samples were detected by using flow cytometry and L SAB immunohistochemistry staining, respectively. The results showed that CyclinD1 and ER expression levels in breast cancer were significantly higher than in benign breast neoplasm (P<0.05). The CyclinDl expression levels in stage I was much lower than in stages II, III, IV (P<0.05). The positive rate of ER was not related with tumor size, lymph node metastasis and TNM stage (P>0.05), but the CyclinD1 expression level in ER (+) group was significantly higher than in ER (-) group (P<0. 05). It was concluded that CyclinDl expression level might be obviously related with the proliferation and metastasis of breast neoplasm and ER.

To study the changes in intratumoral microvessel density (MVD) in hepatocellular carcinoma (HCC) following transcatheter arterial chemoembolization (TACE), MVD in 42 HCC specimens histologically verified was studied by using immunohistochemical method. Of all the specimens, 20 were obtained from the patients treated with surgical resection alone, 22 from those with second stage surgical resection after TACE. The results showed that the MVD in HCC tissues was 53. 4±21. 9 in the TACE group and 27. 6±9. 2 in the single operating group, respectively, with the difference being significant between them (P<0. 001). It was suggested that TACE might contribute to angiogenesis of HCC, possibly due to anoxic stress and ischemia-reperfusion injury.