The content of plasma fibronectin and tissue extractable fibronectin in normal rats was measured with rocket immunoelectrophoresis and ELISA in the present study. The difference in tissue fibronectin distribution in various organs and the correlation between distribution and content of fibronectin have been studied. We suggest that tissue fibronectin may be a complex component. The change in plasma fibronectin reflects a dynamic balance existing between the tissue fibronectin pool and plasma fibronectin pool in normal rats. Plasma fibronectin and tissue fibronectin concentrations are not static, and they can only be maintained in a relatively stable state in normal rats.

A method of prosthetic replacement using total femur with total hip and knee joints is reported. On the surface of the titanium endoprosthesis a ceramic coating was splasma-sprayed to prevent loosening of the prosthesis. By this method the limb can be preserved and the function restored within a short period. It can be used in certain cases of non-malignant tumors and diseases of the bone with extensive destructive lesions of the entire femur. This method was used on 3 patients with good results. The patients operated on were followed up for 32–70 (mean 54) months. The procedures and advantages of the operation are presented.

We observed the effect of pituitrin and phentolamine alone or in combination on wedged hepatic venous pressure (WHVP) and systemic hemodynamics in 28 patients with cirrhosis. The results showed that either of these drugs used separately could lead to reduction in WHVP, each of them could also cause by-effects on systemic hemodynamics. When pituitrin in combination with phentolamine was administered, no change could be found in inferior vena cava pressure, mean arterial pressure, puls rate and cardiac index. This suggested that pituitrin in combination with phentolamine could not only efficaciously decrease WHVP, but also counteract side effects on systemic hemodynamics of each other and improve hepatic microcirculation. Our study provided evidence for the usefulness of the combination of the two drugs in controlling bleeding from esophagus varices.

One hour after the development of myocardial infarction by formation of thrombus, watery extract of Andrographis paniculata Nees (APN) was injected intravenously to 7 dogs for observing the protective effect of this drug on ischemic myocardium. 6 dogs served as control group. In the treated group PGI₂ increased remarkably, synthesis of TXA₂ was inhibited, cAMP in platelets was elevated, CK-MB peak was lowered and appeared earlier, ELT was shortened, release of platelet ß-GT was decreased, platelet maximum aggregation rate was inhibited, the size of ischemie area recorded by epicardial ECG was reduced, the amplitude of ST segment elevation was lower than that in the control group, Q wave appeared in only one dog. Pathologically, the myocardial structure surrounding the initially appearing ischemie area, i.e., the reversiblely damaged area, became relatively normal, while the degree of myocardial degeneration and necrosis in the central part of the ischemie area was mild. These data suggest that APN may limit the expansion of ischemie focus, exert marked protective effect on reversibly ischemie myocardium and demonstrate a weak fibrinolytic action.

A retrospective study of 798 patients from Essen University Medical Hospital during the period 1960–1987 with operation performed due to valvular heart disease was undertaken. Among them were 324 suffering from mitral stenosis, 12 from mitral regurgitation, 462 from mixed mitral valvular diseases. The surgical treatment consisted of commissurotomy in 611 patients, and prosthetic valve replacement in 187 patients.

Follow-up studies showed: Before and after operation, occurrence of embolism was 16.5 vs 8.7 %; status of class of cardiac function (NYHA) from group with commissurotomy was 3.1 vs 2.0; NYHA classification in group of prosthetic replacement was 3.1 vs 1.9; 10 years survival rate after commissurotomy was 95.2 %; after valve replacement it was 80.2 %; early letality rate after operation (at or within 2 months after operation) depended on 1) the type of operations commissurotomy 2.8 %, valve replacement 10.2 %; 2) the state of cardiac function: in class IV it was 12.9 %, in class III 4.6 %; 3) the number of operations: in only operation it was 4.8 %, in reoperations 19.5 %.

A method for quantification of regurgitant volume in mitral regurgitation (MR) was studied, 31 cases of MR were investigated with color Doppler. The reliability of measuring velooity by Doppler and the influence of pressure gradient on MR were assessed and confirmed by experimental device. The results suggested that the best method in quantification of the regurgitant volume was “calibrating method” (r=0.99;P<0.0l), the next to the best was “volume method” (r=o.74;P<0.0l), whereas the regurgitant volume evaluated by multiplication of area, velocity and time was far from the actual regurgitant volume (r=0.47;P>0.05). The velocity of blood flow measured by Doppler is closely correlated to results obtained from experiments (r=0.69)P<0.05). We believe that the ratio of back flow area and average area of left atrium calculated by Helmcke’s method reflects the severity of the disease to a certain degree, but there are many problems unsolved, e.g. the determination of the velocity of blood flow by color Doppler is dependent on the degree of brightness of coloration; the brighter the color, the quicker the speed it represents, whereas the darker coloration speaks for slower speed. Thus the following fact will lead to false judgement: though the area is the same, the regurgitant volume may vary with the brightness of coloration. Furthermore the left auricle will be enlarged consequently following the increase of regurgitant volume, also causing misinterpretation. It is of practical importance to consider some other factors in quantifying the regurgitant volume.

In this study we observed the inhibitory effect of Chinese herbal medicine Ganoderma lucidum (GL) on platelet aggregation in 15 healthy volunteers and 33 patients with atherosclerotic diseases. The results showed that the first and the second phase of aggregation of platelets of the healthy volunteers were obviously inhibited (P<0.0l) when watery soluble extract of GL of different concentrations was added to the platelets in vitro, i. e., the reaction speed of platelet aggregation was slowed down. The inhibitory effect was related to dosage. Platelet aggregation induced by ADP in final concentration of 2 μmol/L and 3 μmol/L was obviously inhibited, after the patients had taken GL 1 g 3 times a day for 2 weeks, the maximum platelet aggregation inhibition rates were then 31.49 % (P<0.0l) and 17.7 % (P<0.0l) respectively. Length and weights (wet and dry) of the extracorporeal thrombi were reduced from 30.05±4.38 mm, 103.9±9.33mg and 44.89±4.79 mg to 20.4±2.33 mm (P<0.05), 85.27±8.77 mg (P<0.0l) and 35.1=±4.5 mg (P<0.0l) respectively after oral administration of GL. The results of our experiments suggested that the Chinese herbal medicine GL may be an effective inhibitory agent of platelet aggregation. However, its mechanism and active principles remain to be further investigated.

From 1987 to 1990 the authors treated 20 cases of spondylolisthesis by an improved operative procedure including excision of the medial part of the superior articular processes of the slipped vertebra, excision of the soft tissue between the ununited isthmi, and excision of the ligamentum flavum between the intervertebral space above the slipped segment. In some instances the lower portion of the lamina over the slipped vertebra should be resected. A U-shaped rod was used to hold sublaminar fixation of two segments above and below the slipped vertebra, with the slipped vertebra spared. Utilizing the U-shaped rod as support, bone strips were placed along the lateral and anterior sides of the rod to bridge the gap between the laminae of the displaced vertebra. Other bone grafts were focused on the facet joints. The patients were allowed ambulation early postoperatively. 19 cases could be evaluated at preliminary follow-up. All showed satisfactory results.

A comparison of the effects of magnesium valproate (MV) and sodium valproate (SV) on the action potential of isolated papillary muscle from guinea pigs and dogs was made in this study. The results in both animals were as follows: MV and SV induced a slight reduction of slope plateau of action potential and prolongation of action potential duration (APD).What is more, APD₅₀ and APD₉₀ were prolonged significantly, thus the ratio of APD₉₀/APD₂₅ was increased. The effective refractory period (ERP) was prolonged significantly. MV or SV showed no effects on action potential amplitude, overshoot, resting potential and phase o upstroke velocity. The above results suggested that MV and SV might play an. antiarrhythmic role and that their effects were analogic. The mechanism of MV and SV inducing significant prolongation of APD₅₀, APD₉₀, and ERP, might be closely related to the slow-down of the velocity of K⁺ efflux during plateau and repolarization of phase 3 by radical of valproate and the slow-down of velocity of K⁺ efflux of repolarizalion of phase 3 in particular. These results showed that there was no difference in SV and MV action on guinea pigs and dogs.

For determination of trace Se we used high-pressure wet digester to pretreat the specimens obtained from the human body. This method is very effective in preventing loss of Se by volatilization which often occurs in the sample pretreatment. We studied the method of using anodic stripping voltammetry at gold-disc electrode to measure the trace Se present in the human body and found it to be the optimal procedure. The detection limitation was about 2x10⁻⁸ g/L, the recovery rate of Se about 96.6±6,7 %

Reported in this paper is the first case of isolation ofPseudalle-Scheria boydii from cerebral spinal fluid of a boy with meningitis in China. Morphology and culture were observed by light microscopy, electron scanning microscopy and transmission electron microscopy. In addition, mycelium antigen prepared by SDS-PAGE was compared with that of the other strain of Scedosporium apiospermum.

Both of the strains showed more than 40 peptide lines. Their molecular weight was very similar. On the CS-930 Dual-Wavelength TLC Scanner the peaks of both antigens showed to lie in nearly the same position. It could be concluded thatPseudallescheria boydii andScedosporium apiospermum belong to the same genus, but they are of different strains and reproductive phases.

100%, 75%, 50%, 25% and 12.5% oxidized dextran T10 (Dex T1o) were used as intermediate carriers for conjugating drug daunorubicin (DNR) and antibody anti-human thymocitic globulin (AHTG), to form different immunoconjugates, AHTG:Dex:DNR. It was demonstrated that the conjugate with 25% oxidized Dex T10 as intermediate carrier linked more DNR molecules than the others. The degree of its substitution was 10–11 moles of DNR per mole of AHTG. Moreover, because the amount to reducing agent sodium borohydride (NaBH₄), required for the reduction reaction, was relatively small, its damaging effect on AHTG and DNR was lessened accordingly.

The antitumor effect of AHTG: Dex: DNR in vitro was tested by using 24-h cytotoxicity assay, with CEM as target cell. Cytotoxic effect of the conjugate was proven and the LD₅₆ was 10.68 μg/ml. However, it showed only slight cytotoxic effect on non-target cell K562. When 10 min cytotoxicity assay was performed to show the specific tumor-killing effect of the conjugate, it revealed an obvious cytotoxic activity toward CEM, with the LD₅₀ being 14.79 μg/ml, but hardly toward K562. These results suggest that AHTG:Dex:DNR possesses specific cytotoxic effect.