The anti-HSV-2 effect of alumen: In vitro and in vivo experimental studies

Abstract

This study investigated the anti-HSV-2 effect of alumen through in vitro and in vivo experiments. Viable cell counting was employed to assess the toxicity of alumen on Vero cells. The inhibition rate of HSV-2 was defined as the cytopathic effect (CPE) of the cells infected with the virus. Alumen suppositories of different concentrations were vaginally applied to the guinea pigs which were then infected with HSV-2 via a vaginal route. The clinical symptoms were observed and the local virus titer calculated. The results showed that alumen had an in vitro anti-HSV-2 effect by means of antiviral duplication, direct killing of the virus, and antiviral adsorption. Alumen suppositories of different concentrations could reduce or completely inhibit HSV-2 infection in guinea pigs. It was concluded that alumen had an in vitro anti-HSV-2 effect through multiple approaches and it could suppress in vivo vaginal HSV-2 infection of guinea pig to some extent.

Keywords

alumen / herpes simplex virus II

References

[1] HuangZ.X., HongT., LiuC.B.. . Medical virology basis and experimental techniques, 1990, Beijing, Science and Technology Publishing House, 83-84

[2] KernE.R., GlasgowL.A., OverallJ.G., et al.. Treatment of experimental herpesvirus infection with phosphononformate and some comparisons with phosphonoacetate. Antimicrob Agents Chemother, 1978, 14: 817-823

[3] DaiS.Z., CheY.C.. Fetation and gential herpes infection. Chin J Prac Gynecol Obstetr (Chinese), 2001, 17(12): 716

[4] LeungD.T., SacksS.L.. Current recommendations for the treatment of genital herpes. Drugs, 2000, 60(6): 1329-1352

[5] NiuK.X.. Two yellow dried alum powder. Shandong J Tradition Chin Med (Chinese), 1996, 15(8): 378

[6] LuoH.Q.. The analysis of persistent rectal prolapse of 30 infants and young children cured by using the dried alum solution injection. Acta academiae medicinae Hubei (Chinese), 1996, 12(3): 289

[7] YangF.J., ZhangQ.P.. The application of alum in Anorectal. New J Tradition Chin Med (Chinese), 2001, 33(12): 41

[8] ChenY.G.. The application of 1% alum solution enema after anal surgery. Modern J Integ Tradition Chin West Med (Chinese), 2003, 12(9): 970-971

[9] National Pharmacopoeia Commission.. . Chinese Pharmacopoeia, Part 1, 20001st edBeijing, Chemical Industry Press, 81

[10] YanM.Z., SongH.Y., ZhangL.M., et al.. Influence of alum on intestinal flora in mice. Chin J Chin Mater Med (Chinese), 1998, 23(12): 743-745

[11] YuJ.W., GaoF.. Study of KAS inhibitory effect on anaerobe and fungus. Yunan J Tradition Chin Med (Chinese), 1990, 7(1): 51

[12] WuE., YangL.M., BaiW.M.. Study on bacteriostatic effect of alum and dried alum in vitro. Acta Academiae Medicinae Neimongol (Chinese), 2007, 29(4): 259-260

[13] YanM.Z., YeW.H., SongH.Y., et al.. Effect of alum on intestinal microecological balance in mice. Chin J Integ Tradition West Med (Chinese), 1999, 19(9): 54

[14] ZhuJ.X., MaiH.Y., HuangJ., et al.. Studies on bacteriostasis of compound Mingfan powder to strains isolated from clinical vaginitis specimens. Chin J Microecol (Chinese), 2005, 17(4): 272-273

[15] GuJ.L.. The treatment of otitis media with borax four flavor of dried alum ear drops. J Chin Commun Doc (Chinese), 2005, 7(6): 57

[16] XueJ.H.. Improved manicure treatment of paronychia with dried alum powder in 81 cases. J Extern Ther Tradition Chin Med (Chinese), 2006, 15(6): 10

[17] LiD.X.. Treatment and nursing care of dried alum powder neonatal omphalitis. Chin J Birth Health & Heredity (Chinese), 2006, 14(10): 79

[18] ZanY.. Topical treatment of dried alum solution in burn wounds with Pseudomonas aeruginosa infection in 86 cases. J Extern Ther Tradition Chin Med (Chinese), 2000, 9(5): 54

[19] WangX.B., HuM., HuangJ.G., et al.. Application of ethanol and potassium alum in resection of the prostate. J Wannan Med Col (Chinese), 1999, 18(3): 225-226

[20] ZhangY.J., QinW.K., WanZ.C.. A primary report on alum liquid used in prostatectomy. J Xinxiang Med Col (Chinese), 1989, 6(2): 98-99

[21] GeR.T.. Acute upper gastrointestinal bleeding treatment with liquid stone alum in 42 cases. Hebei J Tradition Chin Med (Chinese), 1998, 20(2): 131