Neurogenesis of adipose-derived stem cells in hydrogel

Abstract

Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells (ADSCs) were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal (NB) media supplemented with B27, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.

Keywords

adipose-derived stem cells / hydrogel / neurogenesis / tissue engineering

References

[1] AlhadlaqA., TangM., MaoJ.J.. Engineered adipose tissue from human mesenchymal stem cells maintains predefined shape and dimension: implications in soft tissue augmentation and reconstruction. Tissue Eng, 2005, 11(3–4): 556-566

[2] ArrigoniE., LopaS., de GirolamoL., et al.. Isolation, characterization and osteogenic differentiation of adipose-derived stem cells: from small to large animal models. Cell Tissue Res, 2009, 338(3): 401-411

[3] LiH., ZimmerlinL., MarraK., et al.. Adipogenic potential of four distinct cell populations in the stromal vascular fraction of human adipose tissue and implications for soft tissue engineering. J Surg Res, 2010, 158(2): 293

[4] DruryJ.L., MooneyD.J.. Hydrogels for tissue engineering: scaffold design variables and applications. Biomaterials, 2003, 24(24): 433743-43351

[5] TanH., RamirezC.M., MiljkovicN., et al.. Thermosensitive injectable hyaluronic acid hydrogel for adipose tissue engineering. Biomaterials, 2009, 30(36): 6844-6853

[6] KatzJ.S., BurdickJ.A.. Hydrogel mediated delivery of trophic factors for neural repair. Wiley Interdiscip Rev Nanomed Nanobiotechnol, 2009, 1(1): 128-139

[7] The Ministry of Science and Technology of the People’s Republic of China. Guidance Suggestions for the Care and Use of Laboratory Animals. 2006-09-30.

[8] KangS.K., PutnamL.A., YlostaloJ., et al.. Neurogenesis of Rhesus adipose stromal cells. J Cell Sci, 2004, 117(Pt18): 4289-4299

[9] DuttaR.C., DuttaA.K.. Cell-interactive 3D-scaffold: advances and applications. Biotechnol Adv, 2009, 27(4): 334-339

[10] MoroniL., de WijnJ.R., van BlitterswijkC.A.. 3D fiber-deposited scaffolds for tissue engineering: influence of pores geometry and architecture on dynamic mechanical properties. Biomaterials, 2006, 27(7): 974-985

[11] FierzF.C., BeckmannF., HuserM., et al.. The morphology of anisotropic 3D-printed hydroxyapatite scaffolds. Biomaterials, 2008, 29(28): 3799-3806

[12] PatrickC.W.Jr. Adipose tissue engineering: the future of breast and soft tissue reconstruction following tumor resection. Semin Surg Oncol, 2000, 19(3): 302-311

[13] ZukP.A., ZhuM., MizunoH., et al.. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Eng, 2001, 7(2): 211-228

[14] HalvorsenY.D., BondA., SenA., et al.. Thiazolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells: biochemical, cellular, and molecular analysis. Metabolism, 2001, 50(4): 407-413

[15] EricksonG.R., GimbleJ.M., FranklinD.M., et al.. Chondrogenic potential of adipose tissue-derived stromal cells in vitro and in vivo. Biochem Biophys Res Commun, 2002, 290(2): 763-769

[16] XuY., LiuZ., LiuL., et al.. Neurospheres from rat adipose-derived stem cells could be induced into functional Schwann cell-like cells in vitro. BMC Neurosci, 2008, 9: 21