Expression of PirB in normal and injured spinal cord of rats

Abstract

The expression of paired immunoglobulin-like receptor B (PirB) in normal and injured spinal cord of rats was investigated. The SD rat hemi-sectioned spinal cord injury (SCI) model was established. Before and 1, 3, 7, 10 days after SCI, the spinal cord tissues were harvested, and Western blot and immunohistochemistry were used to examine the expression and location of PirB. The results showed that the expression level of PirB in the normal spinal cord of SD rats was low. At the first day after SCI, the expression of PirB was obviously increased, and that in the injured spinal cord from the first day to the 10th day was significantly higher than in the normal spinal cord. The positive expression of PirB in neurons from different regions of gray matter of the injured spinal cord was seen. It was concluded that the expression of PirB in the normal spinal cord of rats was low. The expression of PirB in SCI was significantly increased till at least the 10th day.

Keywords

paired immunoglobulin-like receptor B / spinal cord / injury / rat

References

[1] NiederostB., OertleT., FritscheI., et al.. Nogo-A and myelin-associated glycoprotein mediate neurite growth inhibition by antagonistic regulation of RhoA and Rac I. J Neurosci, 2002, 22(23): 10 368-10 376

[2] KimJ.E., LiuB.P., ParkJ.H., et al.. Nogo-66 receptor prevents raphespinal and rubrospinal axon regeneration and limits functional recovery from spinal cord injury. Neuron, 2004, 44(3): 439-451

[3] ZhangB., AtwalJ., HoC., et al.. Genetic deletion of the Nogo receptor does not reduce neurite inhibition in vitro or promote corticospinal tract regeneration in vivo. Proc Natl Acad Sci USA, 2005, 102(4): 1205-1210

[4] StewardO., SharpK., YeeK.M., et al.. A re-assessment of the effects of a Nogo-66 receptor antagonist on regenerative growth of axons and locomotor recovery after spinal cord injury in mice. Exp Neurol, 2008, 209(2): 446-468

[5] AtwalJ.K., Pinkston-GosseJ., SykenJ., et al.. PirB is a functional receptor for myelin inhibitors of axonal regeneration. Science, 2008, 322(5903): 967-970

[6] SykenJ., GrandpreT., KanoldP.O., et al.. PirB restricts ocular-dominance plasticity in visual cortex. Science, 2006, 313(5794): 1795-1800

[7] KubagawaH., BurrowsP.D., CooperM.D.. A novel pair of immunoglobulin-like receptors expressed by B cells and myeloid cells. Proc Natl Acad Sci USA, 1997, 94(10): 5261-5266

[8] YamashitaY., FukutaD., TsujiA., et al.. Genomic structures and chromosomal location of p91, a novel murine regulatory receptor family. J Biochem, 1998, 123(2): 358-368

[9] BléryM., KubagawaH., ChenC.C., et al.. The paired Ig-like receptor PIR-B is an inhibitory receptor that recruits the protein-tyrosine phosphatase SHP-1. Proc Natl Acad Sci U S A, 1998, 95(5): 2446-2451

[10] MaedaA., KurosakiM., OnoM., et al.. Requirement of SH2-containing protein tyrosine phosphatases SHP-1 and SHP-2 for paired immunoglobulin-like receptor B (PIR-B)-mediated inhibitory signal. J Exp Med, 1998, 187(8): 1355-1360

[11] YamashitaY., OnoM., TakaiT.. Inhibitory and stimulatory functions of paired Ig-like receptor (PIR) family in RBL-2H3 cells. J Immunol, 1998, 161(8): 4042-4047

[12] TakaiT.. A novel recognition system for MHC class I molecules constituted by PIR. Adv Immunol, 2005, 88: 161-192

[13] PereiraS., ZhangH., TakaiT., et al.. The inhibitory receptor PIR-B negatively regulates neutrophil and macrophage integrin signaling. J Immunol, 2004, 173(9): 5757-5765

[14] ZhangH., MengF., ChuC.L., et al.. The Src family kinases Hck and Fgr negatively regulate neutrophil and dendritic cell chemokine signaling via PIR-B. Immunity, 2005, 22(2): 235-246

[15] PereiraS., LowellC.. The Lyn tyrosine kinase negatively regulates neutrophil integrin signaling. J Immunol, 2003, 171(3): 1319-1327

[16] ChanC.S., WeeberE.J., KurupS., et al.. Integrin requirement for hippocampal synaptic plasticity and spatial memory. J Neurosci, 2003, 23(18): 7107-7116

[17] ChunD., GallC.M., BiX., et al.. Evidence that integrins contribute to multiple stages in the consolidation of long term potentiation in rat hippocampus. Neuroscience, 2001, 105(4): 815-829

[18] KramárE.A., BernardJ.A., GallC.M., et al.. Alpha3 integrin receptors contribute to the consolidation of long-term potentiation. Neuroscience, 2002, 110(1): 29-39

[19] KramárE.A., BernardJ.A., GallC.M., et al.. Integrins modulate fast excitatory transmission at hippocampal synapses. J Biol Chem, 2003, 278(12): 10722-10730

[20] HenschT.K.. Critical period regulation. Annu Rev Neurosci, 2004, 27: 549-579