Separation and identification of flavonoids from fistular onion stalk (Allium fisturosum L. var. Caespitosum Makio)

Abstract

The chemical constituents of fistular onion stalk obtained by supercritical CO₂ extraction were separated and purified by silica gel and sephadex LH-20 gel column chromatography and the preparative TLC method and four flavonoids were obtained. On the basis of the spectral data, they were structurally identified as (+)-catechin, (−)-epicatechin, astragalin, and 3-O-β-D(2-O-β-D-glucopyranosyl)-glucopyranosides of kaempferol.

Keywords

fistular onion stalk / allium fisturosum / supercritical CO₂ extraction / chemical constituents / flavonoids

References

[1] AlfonsoC., ErnestoF., VirginiaL., et al.. The flavonoids of Allium neapolitanum. Phytochemistry, 1997, 44(5): 949

[2] AlfonsoC., VincenzoD.F., ErnestoF., et al.. The flavonoids of Allium ursinum. Phytochemistry, 1996, 41(2): 531

[3] GuoJ., ZhangJ.M., TuX., et al.. Effect of extraction of fistular onion stalk on NO and eNOS in cultured human umbilical vein endothelium cells. Chin J Integr Med Cardiocerebrovasc Dis (Chinese), 2008, 6(1): 34-35

[4] SuM.M., YuX.F., SuiD.Y., et al.. Effects of TFAD on blood-liquid metabolism in experimental hyperlipo proteinemia rats. Chin Pharmacol Bull (Chinese), 2007, 23(12): 1593-1597

[5] ShiZ.H., ZhangJ.M., ZhengY.Q., et al.. Influences of fresh fistular onion stalker extract on preventing nonalcoholic fatty liver. Chin J Integr Tradit West Med Liver Dis (Chinese), 2009, 19(4): 229-231

[6] BlockE., AhmaS., JainM.K., et al.. (E, Z)-Ajoene: A potent antithrombotic agent from garlic. J Am Chem Soc, 1984, 106(26): 8295-8296

[7] BernhardR.A.. Distribution studies of sulphur compounds in Allium. Phytochemistry, 1970, 9: 2019-2027

[8] HayashiT.. Anion acids in the garlic. Biosci Biotechno Biochem, 1993, 57(1): 162-163

[9] MackenzieI.A., FernsD.A.. The composition of volatiles from different parts of Allium tuberosum plants. Phytochemistry, 1977, 16: 763-764

[10] LiuY.J., LiuL.J., YangY.H.. Study on the chemical constituent of Allium cepa L. var agrogatum don. Zhong Caoyao (Chinese), 2000, 31(5): 333-334

[11] AlfonsoC., ErnestoF., VirgimaL., et al.. The flavonoids of Allium nrapolitanum. Phytochemistry, 1997, 44(5): 949

[12] MartinM.J., La CasaC., Alarcon de lastraC., et al.. Antioxidant mechanisms involved in gastroprotective effects of quercetin. Z Naturforsch C, 1998, 53(1–2): 82-88

[13] HansenR.K., OesterreichS., LemieuxP., et al.. Quercetin inhibits heat shock protein induction but not heat shock factor DNA-binding in human breast carcinoma cells. Biochem Biophys Res Commun, 1997, 239(3): 851-856

[14] BeilW., BirkholzC., SewingK.F.. Effects of flavonoids on parietal cell acid secretion, gastric mucosal prostaglandin production and Helicobacter pylori growth. Arzneimittelforschung, 1995, 45(6): 697

[15] LiW.L., LuQ.B., HeQ.L., et al.. Effect of calcium and extraction of cristata flavonoid on disuse osteoporosis in rats. J Huazhong Univ Sci Tech [Health Sci] (Chinese), 2003, 32(6): 601-603

[16] ZhouY.B., ZhangC.C., FuQ.Q., et al.. Components analyzing in essential oil extracted with supercritical CO₂ from fistular onion stalk by GC-MS. Herald Med(Chinese), 2009, 28(2): 158-159

[17] ZhaoJ.Q., YiY.M., XuY.. Measurement of the total sapon in in fistular onion bulb by vanillin chromatometry. Herald Med(Chinese), 2007, 26(9): 1075-1076