Change profiles in matrix metalloproteinase-2 and -9 in induced endometriosis in mice

Abstract

To examine the changes in matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9) in the development and progression of endometriosis, real time quantitative polymerase chain reaction, enzyme-linked immunoabsorbent assay and gelatin zymography were employed to determine the mRNA and protein levels and activities of MMP-2 and MMP-9 from the first day to the 21^st day after the induction in mice with induced endometriosis (experimental group) and sham-operated animals (controls). The results showed that the mRNA and protein levels and activities of the MMP-2 and MMP-9 were significantly increased on the first day after the induction and the level of MMP-2 stayed at a level higher than that in the control group. MMP-9 had two or three peaks during the 21 days, taking place at day 1, 4 and 15. It is concluded that the changes in the MMP-2 and MMP-9 might be involved in pathogenesis of endometriosis.

Keywords

matrix metalloproteinase / endometriosis / animal model

References

[1] ChenQ.H., QuJ.Y., XuY.Y., et al.. Expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in ectopic and eutopic endometrium. Chin J Obstet Gynecol (Chinese), 2004, 39(12): 809-812

[2] LiT., LiY.G., PuD.M.. Matrix metalloproteinase-2 and -9 expression correlated with angiogenesis in human adenomyosis. Gynecol Obstet Invest, 2006, 62: 229-35

[3] VincentL., ElisabethBoichot.GascheY., CopinJ.C.. Matrix metallo-proteinases in tissue remodelliing and inflammation. Matrix metalloproteinases and inflammatory diseases of the central nervous system, 2008, Germany Berlin, Die Deutche Bibliothek: 123-152

[4] ChenQ.H., ZhouW.D., PuD.M., et al.. The inhibitory effect of 15-R-LXA4 on experimental endometriosis. EJOG, 2009, 145(2): 200-204

[5] CaoZ.Y.GaoY.. Chinese Gynecology and Obstetrics. Pathology of Ectopic Endometrium, 2004, Beijing, People’s Medical Publishing House: 1486-1488

[6] LangJ.H.. Researches and hypostheses on ectopic endometrium. Chin J Obstet Gynecol (Chinese), 2003, 38(8): 478-480

[7] SternlichtM., WerbZ.. How matrix metalloproteinases regulate cell behavior. Annu Rev Cell Dev Biol, 2001, 17: 463-516

[8] Gilabert-EstellésJ., RamónL.A., EspañaF., et al.. Expression of angiogenic factors in endometriosis: relationship to fibrinolytic and metalloproteinase systems. Hum Reprod, 2007, 22(8): 2120-2127

[9] ChenQ.H., ZhouW.D., SuZ.Y., et al.. Change of pro-inflammatory cytokines follows certain patterns after induction of endometriosis in a mouse model. Fertil Steril, 2010, 93(5): 1448-1454

[10] ParksW.C., WilsonC., Lopez-BoadoY.S.. Matrix metalloproteinases as modulators of inflammation and innate immunity. Nat Rev Immunol, 2004, 4(8): 617-629

[11] MulayimN., SavluA., Guzeloglu-KayisliO., et al.. Regulation of endometrial stromal cell matrix metalloproteinase activity and invasiveness by interleukin-8. Fertil Steril, 2004, 81(Suppl1): 904-911

[12] ManY.C., LiuY., XieW., et al.. Effect of blockage of Wnt/β-catenin signaling pathway on activation eutopic endometrial stromal cell of patients with endometriosis by 17β-estradiol treatment. Acta Med Univ Sci Technol Huazhong (Chinese), 2009, 38(4): 421-424,429

[13] MottJ.D., WerbZ.. Regulation of matrix biology by matrix metalloproteinases. Curr Opin Cell Biol, 2004, 16(5): 558-564