Millimeter-wave exposure promotes the differentiation of bone marrow stromal cells into cells with a neural phenotype

Abstract

This study investigated the ability of millimeter-wave (MMW) to promote the differentiation of bone marrow stromal cells (BMSCs) into cells with a neural phenotype. The BMSCs were primarily cultured. At passage 3, the cells were induced by β-mercaptoethanol (BME) in combination with MMW or BME alone. The expressions of nucleostemin (NS) and neuron-specific enolase (NSE) were detected by immunofluorescent staining and Western blotting respectively to identify the differentiation. The untreated BMSCs predominately expressed NS. After induced by BME and MMW, the BMSCs exhibited a dramatic decrease in NS expression and increase in NSE expression. The differentiation rate of the cells treated with BME and MMW in combination was significantly higher than that of the cells treated with BME alone (P<0.05). It was concluded that MMW exposure enhanced the inducing effect of BME on the differentiation of BMSCs into cells with a neural phenotype.

Keywords

bone marrow stromal cells / β-mercaptoethanol / millimeter-wave / nucleostemin / neuron specific enolase

References

[1] HarveyC., FrenchP.W.. Effects on protein kinase C and gene expression in a human mast cell line, HMC-1, following microwave exposure. Cell Biol Int, 2000, 23: 739-748

[2] ThornberryN.A., LazebnikY.. Caspases: enemies within. science, 1998, 281: 1312-1316

[3] WangZ.H., LuS.J., HuH.S., et al.. Channel millimeter-wave conduction with radiotherapy for the treatment of malignant tumors. Chin J Phys Med Rehabil (Chinese), 1997, 19(1): 2-5

[4] RongL., SunD.Y., PangH.F., et al.. Effect of millimeter wave on rat hepatic tumor induced by diethylnitrosamine. J Fudan Univ: Med Sci (Chinese), 2001, 28(2): 133-135

[5] ReynoldsB.A., WeissS.. Generation of neurons and astrocytes from isolated cell of adult mammalian central nervous system. Science, 1992, 225: 1707-1710

[6] FriendensteinA.J., GorskajaJ.F., KulaginaN.N.. Fibmblast precursors in normal and irradiated mouse hematopoietie organs. Exp Hematol, 1976, 4(5): 267-274

[7] PereiraR.F., HalfordK.W., O’HaraM.D., et al.. Cultured adherent cells from marrow can serve as long-lasting precursor cells for bone, cartilage and lung in irradiated mice. Proc Natl Acad of Sci USA, 1995, 92(11): 4857-4861

[8] MengF.G., WuC.Y., ZhuS.G., et al.. Neuronal development of bone marrow stromal stem cells induced by arsenious acid. Chin J Exp Surg (Chinese), 2005, 22(9): 1120-1122

[9] ChenX.P., RuanX.Z., ZhangQ.L., et al.. Effect of NGF and BME on differentiation of BMSCs into neurons-like cells. Chin J Mod Med (Chinese), 2004, 14(15): 20-23

[10] WoodburyD., SchwarzE.J., ProckopD.J., et al.. Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res, 2000, 61(4): 364-370

[11] WetzelB.J., NindlG., VesperD.N., et al.. Electromagnetic field effects: changes in protein phosphorylation in the Jurkat E6.1 cell line. Biomed Sci Instrum, 2001, 37: 203-208

[12] AlekseevS.I., ZiskinM.C.. Effects of millimeter waves on ionic currents of Lymnaea neurons. Bioelectromagnetics, 1999, 20(1): 24-33

[13] YangZ.H., ChenJ.J., LiuL., et al.. The effect of millimeter wave irradiation on the axon regeneration after peripheral nerve injury. Chin J Phys Med Rehabil (Chinese), 2005, 27(7): 395-397

[14] BervarM.. Effect of weak, interrupted sinusoidal low frequency magnetic field on neural regeneration in rats: functional evaluation. Bioelectromagnetics, 2005, 26(5): 351-356

[15] WoodM., WillitsR.K.. Short-duration, DC electrical stimulation increases chick embryo DRG neurite outgrowth. Bioelectromagnetics, 2006, 27(4): 328-331

[16] ZhangY., DingJ., DuanW., et al.. Influence of pulsed electromagnetic field with different pulse duty cycles on neurite outgrowth in PC12 rat pheochromocytoma cells. Bioelectromagnetics, 2005, 26(5): 406-411

[17] Prina-MelloA., FarrellE., PrendergastP.J., et al.. Influence of strong static magnetic fields on primary cortical neurons. Bioelectromagnetics, 2006, 27(1): 35-42

[18] YangL.Y., HuangT.H., MaL.. Bone marrow stromal cells express neural phenotypes in vitro and migrate in brain after transplantation in vivo. Biomed Environ Sci, 2006, 19(5): 329-335

[19] GaoY.J., QianW., WangB.H., et al.. Differentiation potential of bone marrow stromal cells to enteric neurons in vitro. Chin J Dig Dis (Chinese), 2006, 7(3): 156-163

[20] ScintuF., RealiC., PillaiR., et al.. Differentiation of human bone marrow stem cells into cells with a neural phenotype: diverse effects of two specific treatments. BMC Neurosci, 2006, 7: 14

[21] MaH., PedersonT.. Nucleostemin: a multiplex regulator of cell-cycle progression. Trends Cell Biol, 2008, 18(12): 575-579

[22] MengL., LinT., TsaiR.Y.. Nucleoplasmic mobilization of nucleostemin stabilizes MDM2 and promotes G2-M progression and cell survival. J Cell Sci, 2008, 121(Pt24): 4037-4046

[23] NormileD.. Cell proliferation. Common control for cancer, stem cells. Science, 2002, 298(5600): 1869

[24] TsaiR.Y., McKayR.D.. A nucleolar mechanism controlling cell proliferation in stem cells and cancer cells. Genes Dev, 2002, 16(23): 2991-3003