Endothelial nitric oxide synthase traffic inducer in the umbilical vessels of the patients with pre-eclampsia

Abstract

The expression of endothelial nitric oxide synthase traffic inducer (NOSTRIN) was examined in the umbilical vessels of the patients with pre-eclampsia (PE) to explore its possible role in the pathogenesis of PE. The NOSTRIN mRNA in umbilical tissues was determined by RT-PCR. The eNOS activity in umbilical vessels was spectrophotometrically detected. NO₂⁻/NO₃⁻, the stable metabolic end products of NO, was measured by using nitrate reductase. RT-PCR showed that the expression level of NOSTRIN was significantly higher in women with PE than in the normal group (P<0.01). The activity of eNOS was significantly decreased in PE group [(12.83±3.61) U/mg] than in normal group [(21.72±3.83) U/mg] (P<0.01). The level of NO₂⁻/NO₃⁻ in PE patients (27.53±7.48) μmol/mg was significantly lower than that of normal group (54.27±9.53) μmol/mg (P<0.01). The significant negative correlation existed between the expression of NOSTRIN and the activity of eNOS in umbilical vessels of women with PE (r=−0.58, P<0.01). It was concluded that the level of NOSTRIN expression was increased in umbilical vessel of women with PE, indicating that it may be involved in the pathogenesis of PE.

Keywords

OSTRIN / pre-eclampsia / umbilical vessel / eNOS

References

[1] ZimmermannK., OpitzN., DedioJ., et al. . NOSTRIN: a protein modulating nitric oxide release and subcellular distribution of endothelial nitric oxide synthase. Proc Natl Acad Sci USA, 2002, 99(26): 17167-17172

[2] LeJ.. Gynecology, 20046th editionBeijing, People’s Heatlth Hopstpials: 333-765

[3] ShaamashA.H., ElsnosyE.D., MakhloufA.M.. Maternal and fetal serum nitric oxide (NO) concentrations in normal pregnancy, pre-eclampsia and eclampsia. Int J Gynaecol Obstet, 2000, 68(3): 207-214

[4] SeligmanS.P., BuyonJ.P., ClancyR.M.. The role of nitric oxide in the pathogenesis of preeclampsia. Am J Obstet Gynecol, 1994, 171(4): 944-948

[5] VarA., YildirimY., OnurE., et al. . Endothelial dysfunction in preeclampsia. Increased homocysteine and decreased nitric oxide levels. Gynecol Obstet Invest, 2003, 56(4): 221-224

[6] RobertsJ.M.. Endothelial dysfunction in preeclampsia. Semin Reprod Endocrinol, 1998, 16(1): 5-15

[7] XiangW.P., ChenH.P., XuX.Y.. Clone and Expression of NOSTRIN Gene in Human Umbilical Vein Endothelial Cell. Adv Mod Gynecol (Chinese), 2004, 13(5): 336-339

[8] XiangW.P., ChenH.P.. Study on clone and express endothelial nitric oxide synthase traffic inducer protein. Chin J Birth Health Hered (Chinese), 2005, 13(1): 24-26

[9] XiangW.P., ChenH.P., XuX.Y., et al. . Expression of endothelial nitric oxide synthase traffic inducer in the placentas of women with preeclampsia. Int J Gynecol Obstet (Chinese), 2005, 89(2): 103-107

[10] XiangW.P., ChenH.P., GuoY.Z.. Study on the expression of endothelial nitric oxide synthase traffic inducer in the placenta of pregnancy induced hypertension syndrome. Matern Child Health Care China (Chinese), 2006, 21(2): 228-230