The effect of hypercapnic acidosis preconditioning on rabbit myocardium

Abstract

This study observed the protective effect of hypercapnic acidosis preconditioning on rabbit heart suffered from ischemia-reperfusion injury. Hypercapnic acidosis was established in animals with mechanical hypoventilation before ischemia-reperfusion. Thirty-two rabbits were randomly divided into 4 groups, with each having 8 aminals in term of the degree of acidification: hypercapnic acidosis group A (group A), hypercapnic acidosis group B (group B), hypercapnic acidosis group C (group C), ischemia and reperfusion group (group IR). Animals in group IR were ventilated normally (tidal volume: 15 mL/kg, breathing rate 35 bpm). The PETCO₂ was maintained at the level of 40–50 mmHg for 30 min. Animals in groups A, B, C received low-frequency, low-volume ventilation to achieve hypercarbonic acidosis and the target levels of PETCO₂ were 75–85,65–75, 55–65 mmHg, respectively, with levels being maintained for 5 min. The animals then were ventilated normally to lower PETCO₂ to 40–50 mmHg. The left anterior branch artery of all the animals was ligated for 30 min and reperfused for 180 min. Then the infarct size was calculated. The cardiomyocytes were morphologically observed and ECG and hemodynamics were monitored on continuous basis. Acid-base balance was measured during procedure. Our results showed that the infarct size was (48.5±11.5)% of the risk area in the control group and (42.4±7.9)% in group C (P>0.05). Mean infarct size was significantly smaller in group B (34.5%±9.4%) (P<0.05 vs control group) and group A (31.0%±9.1%) (P<0.01 vs control group). It is concluded that HA-preconditioning can effectively protect the myocardium.

Keywords

hypercapnic acidosis / preconditioning / heart / infarct size