The effect of premixed schedule on the crystal formation of calcium phosphate cement-chitosan composite with added tetracycline

Abstract

In this study, calcium phosphate cements (CPC) were prepared by mixing cement powders of tetracalcium phosphate (TTCP) with a cement liquid of phosphate acid saline solution. Tetracycline (TTC)-CPC, chitosan-CPC and chitosan-TTC-CPC were investigated with different premixed schedule. It was demonstrate that both TTC and chitosan worked on the phase transition and crystal characteristics. TTCP mixed with phosphate acid saline solution had similar features of Fourier transform-infrared spectrometry (FT-IR) no matter it was mixed with chitosan or TTC or both. TTC premixed with cement liquid or powder had significant different features of FT-IR and 876 cm^–1 seemed to be a special peak for TTC when TTC was premixed with cement liquid. This was also supported by XRD analysis, which showed that TTC premixed with cement liquid improved phase transition of TTCP to OCP. Chitosan, as organic additive, regulates the regular crystal formation and inhibits the phase transition of TTCP to OCP, except when it is mingled with cement liquid premixed with TTC in field scanning electron microscope. It was concluded that the premixed schedule influences the crystal formation and phase transition, which may be associated with its biocompatibility and bioactivities in vivo.

Keywords

calcium phosphate cement / chitosan / tetracycline / tetracalcium phosphate / octacacium phosphate

References

[1] XuH. H. K., QuinnJ. B., TakagiS., et al.. Processing and properties of strong and non-rigid calcium phosphate cement. J Dent Res, 2002, 81(3): 219-224

[2] BrowW. E., ChowL. C.. Singular points in the chemistry of teeth. J Dent Res, 1975, 54: 74

[3] OomsE. M., EgglezosE. A., WolkeJ. G. C., et al.. Soft-tissue response to injectable calcium phosphate cements. Biomaterials, 2003, 24: 749-757

[4] RatierA., FrecheM., LacoutJ. L., et al.. Behavior of an injectable calcium phosphate cement with added TTC. Int J Pharm, 2004, 274: 261-268

[5] AtsuroY., SatoruY., TakaoK., et al.. Developemntof calcium phosoahte cement using chitosan and citric acid for bone substitute materials. Biomaterial, 2002, 23: 1091-1101

[6] WangX., MaJ., WangY., et al.. Structural characterization of phosphorylated chitosan and their applications as effective additives of calcium phosphate cements. Biomaterial, 2001, 22: 2247-2255

[7] WangX., MaJ., FengQ., et al.. In vivo evaluation of S-chitosan enhanced calcium phosphate cements. J Bioactive and Compatible Polymers, 2003, 18(4): 259-271

[8] PangE. K., PaikJ. W., KimS. K., et al.. Effects of chitosan on human periodontal ligament fibroblasts in vitro and on bone formation in rat calvarial defects. J Periodontol, 2005, 76(9): 1526-1533

[9] TakagiS., ChowL. C., HirayamaS., et al.. Properties of elastomeric calcium phosphate cement-chitosan composites. Dental materials, 2003, 19: 797-804

[10] TakagiS., ChowL. C., IshikawaK., et al.. Formation of hydroxyapatite in new calcium phosphate cements. Biomaterials, 1998, 19: 1593-1599

[11] WangJ., de BoerJ., de GrootK.. Preparation and characterization of electrodeposited calcium phosphate/chitosan coating on Ti6Al4V plates. J Dent Res, 2004, 83(4): 296-301

[12] GuoD. G., XuK. W., SunH. L., et al.. Physicochemical properties of TTCP/DCPA system cement formed in physiological saline solution and its cytotoxicity. J Biomed Mater Res, 2006, 77(2): 313-323

[13] YokoyamaA., YamamotoS., KawasakiT., et al.. Development of calcium phoshpahte cement using chitosan and citric acid for bone substitute materials. Biomaterials, 2002, 23: 1091-1101

[14] DriessensF. C. M., BoltongM. G., BermudezO., et al.. Effective formulations for preparation of calcium phosphate bone cements. J Mater Sci Mater Med, 1994, 5: 164-170

[15] IshikawaK., EanesE. D.. The hydrolysis of anhydrous dicalcium phosphate into hydroxyapatite. J Dent Res, 1993, 72(2): 474-80

[16] PangE. K., PaikJ. W., KimS. K., et al.. Effects of chitosan on human periodontal ligament fibroblasts in vitro and on bone formation in rat calvarial defects. J Periodontol, 2005, 76(9): 1526-1530