Effect of quercetin on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells

Abstract

The effect of quercetin (Que) on proliferation and apoptosis of human nasopharyngeal carcinoma HEN1 cells was investigated. Inhibition rate of quercetin on HEN1 was assayed by MTT method, apoptosis by flow cytometry (FCM), and the caspase-3 expression of each group by colorimetry set respectively. Quercetin inhibited HEN1 cells in in a dose-(r=0.709, P<0.01) and time-dependent manner (r=0.703, P<0.01). The ratio of apoptotic and necrosis cells was increased in the cells treated with quercetin. Cell cycle was specificly arrested in G₂/M phase. Apoptosis cusp was revealed by FCM. The activity of caspase-3 was significantly up-regulated in 5 groups treated with quecetin as compared with control group (P<0.05). It was concluded that the growth inhibition of quercetin was highly related to cell cycle arrest at the G₂/M phase and induction of caspase-dependent apoptosis in human nasopharyngeal carcinoma HEN1 cells.

Keywords

quercetin / HEN1 cells / apoptosis / cell cycle / caspase-3

References

[1] ManachC., ScalbertA., MorandC., et al.. Polyphenols: food sources and bioavailability. Am J Clin Nutr, 2004, 79(5): 727-747

[2] LiuY., ZhangS., CaiY.. Cytoprotective effects of selenium on cadmium-induced LLC-PK1 cells apoptosis by activating JNK pathway. Toxicol In Vitro, 2007, 21(4): 677-684

[3] CheongE., IvoryK., DolemanJ., et al.. Synthetic and naturally occurring COX-2 inhibitors suppress proliferation in a human oesophageal adenocarcinoma cell line (OE33) by inducing apoptosis and cell cycle arrest. Carcinogenesis, 2004, 25(10): 1945-1952

[4] NguyenT. T., TranE., NguyenT. H., et al.. The role of activated MEK-ERK pathway in quercetin-induced growth inhibition and apoptosis in A549 lung cancer cells. Carcinogenesis, 2004, 25(5): 647-659

[5] SalucciM., StivalaL. A., MaianiG., et al.. Flavonoids uptake and their effect on cell cycle of human colonadenocarcinoma cells (Cacco2). Br J Cancer, 2002, 86(10): 1645-1651

[6] ChoiJ. A., KimJ. Y., LeeJ. Y., et al.. Induction of cell cycle arrest and apoptosis in human breast cancer cells by quercetin. Int J Oncol, 2001, 19(4): 837-844

[7] YuanH. Q., YangL. L., JiangA. L., et al.. Inhibitory effects of quercetin on proliferation of prostate cancer cells and activity of transcription factor Sp1. Chin J Biochem Mol Biol (Chinese), 2005, 21(5): 673-678

[8] KuoH. M., HoH. J., ChaoP. D., et al.. Quercetin glucuronides inhibited 2-aminofluorene acetylation in human acute myeloid HL-60 leukemia cells. Phytomedicine, 2002, 9(7): 625-631

[9] CuiW.. Principle of cell cycle analysis by PI staining. Chin J Lab Med (Chinese), 2004, 27(3): 151

[10] CaoY. X., JinW. L., JinB. Q., et al.. A new method specified for detecting apoptosis and cell cycles. Chin J Med Res (Chinese), 2003, 3(12): 234-236

[11] YangJ. H., HsiaT. C., KuoH. M., et al.. Inhibition of lung cancer cell growth by quercetin glucuronides via arrest G2/M and induction of apoptosis. Drug Metab Dispos, 2006, 34(2): 296-304