Local Th1/Th2 cytokine expression in experimental murine vaginal candidiasis

Abstract

In order to investigate the expression of Th1 and Th2 cytokines in the vaginal candidiasis caused by Candida, the fungal vaginitis model was established in female ICR mice by intravaginal inoculation of suspension of C. albicans after the animals were pretreated with estradiol. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to detect the expression of IL-2, IL-4, IL-10 and TGF-β1 in the vagina in the mice of different groups at different time points after the beginning of the experiment. The average expression level of IL-2 mRNA in group D (estrogen-treated mice) was significantly higher than that in groups H (estrogen-untreated mice) and I (control group) on the day 2. The average expression level of IL-4 mRNA in group D was significantly higher than that in groups I and H on the day 5. The average expression level of IL-10 mRNA in group D was significantly higher than that in groups H and I from day 7 to 11. The average expression level of TGF-β1 mRNA in group D was significantly higher than that in groups H and I at all time points. It was concludes that the high-level expression of IL-2 mRNA during early infection was associated with clearance of mucosal C. albicans, and the high-level expression of IL-10 mRNA during late stage of the infection was related to susceptibility to infection. TGF-β1 may play a predominant role when the virtual absence of changes in other Th-type cytokines during infection.

Keywords

Candida albicans / vaginal lavage / murine model / Th1/Th2 cytokine

References

[1] FidelP. L.Jr, WolfN. A., KuKurugaM. A.. T lymphocytes in the murine vaginal mucosa are phenotypically distinct from those in the periphery. Infect Immun, 1996, 64: 3793-3799

[2] FidelP. L.Jr, LynchM. E., Redondo-LopezV., et al.. Systemic cell-mediated immune reactivity in women with recurrent vulvovaginal candidiasis (RVVC). J Infect Dis, 1993, 168: 1458-1465

[3] FidelP. L.Jr, LynchM. E., ConawayD. H., et al.. Mice immunized by primary vaginal C. albicans infection develop acquired vaginal mucosal immunity. Infect Immun, 1995, 63: 547-553

[4] FidelP. L.Jr, LynchM. E., SobelJ. D.. Effects of preinduced Candida-specific systemic cell-mediated immunity on experimental vaginal candidiasis. Infect Immun, 1994, 62: 1032-1038

[5] RomaniL., PuccetT. I. P., BistoniF.. RomagnaniS.. Biological role of Th cell subsets in candidiasis. Th1 and Th2 cells in Health and Disease, 1996, Farmington, CT, Karger: 114-137

[6] CenciE., MencacciA., SpaccapeloR., et al.. T helper cell type 1 (Th1)-and Th2-like responses are present in mice with gastric candidiasis but protective immunity is associated with Th1 development. J Infect Dis, 1995, 171: 1279-1288

[7] MacherA. M.. The pathology of AIDS. Public Health Report, 1988, 103: 246-254

[8] CliftR. A.. Candidiasis in the transplant patient. Am J Med, 1984, 77(Suppl.4D): 34-38

[9] KleinR. S., HarrisC. A., SmallC. B., et al.. Oral candidiasis in high-risk patients as the initial manifestation of the acquired immunodeficiency syndrome. N Engl J Med, 1984, 311: 354-357

[10] TaylorB. N., SaavedraM., FidelP. L.. Local Th1/Th2 cytokine production during experimental vaginal candidiasis: potential importance of transforming growth factor-β1. Med Mycol, 2000, 38: 419-431