Identification and characterization of peptide mimics of blood group A antigen

Zhaoming Tang , Lin Wang , Lihua Hu , Yirong Li , Tianpen Cui , Juan Xiong , Lifang Dou

Current Medical Science ›› 2008, Vol. 28 ›› Issue (28) : 222 -226.

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Current Medical Science ›› 2008, Vol. 28 ›› Issue (28) : 222 -226. DOI: 10.1007/s11596-008-0228-0
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Identification and characterization of peptide mimics of blood group A antigen

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Abstract

In order to investigate peptide mimics of carbohydrate blood group A antigen, a phage display 12-mer peptide library was screened with a monoclonal antibody against blood group A antigen, NaM87-1F6. The antibody-binding properties of the selected phage peptides were evaluated by phage ELISA and phage capture assay. The peptides were co-expressed as glutathione S-transferase (GST) fusion proteins. RBC agglutination inhibition assay was performed to assess the natural blood group A antigen-mimicking ability of the fusion proteins. The results showed that seven phage clones selected bound to NaM87-1F6 specifically, among which, 6 clones bore the same peptide sequence, EYWYCGMNRTGC and another harbored a different one QIWYERTLPFTF. The two peptides were successfully expressed at the N terminal of GST protein. Both of the fusion proteins inhibited the RBC agglutination mediated by anti-A serum in a concentration-dependent manner. These results suggested that the fusion proteins based on the selected peptides could mimic the blood group A antigen and might be used as anti-A antibody-adsorbing materials when immunoabsorption was applied in ABO incompatible transplantation.

Keywords

amino acid sequence / blood group A antigen / hemagglutination test / molecular mimicry / peptide library

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Zhaoming Tang, Lin Wang, Lihua Hu, Yirong Li, Tianpen Cui, Juan Xiong, Lifang Dou. Identification and characterization of peptide mimics of blood group A antigen. Current Medical Science, 2008, 28(28): 222-226 DOI:10.1007/s11596-008-0228-0

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References

[1]

SawadaT., FuchinoueS., TeraokaS.. Successful Al-to-O ABO-incompatible kidney transplantation after a preconditioning regimen consisting of anti-CD20 monoclonal antibody infusions, splenectomy, and double-filtration plasmapheresis. Transplantation, 2002, 74: 1207-1210

[2]

NordenG., BriggsD., CockwellP., et al.. ABO-incompatible live donor renal transplantation using blood group A/B carbohydrate antigen immunoadsorption and anti-CD20 antibody treatment. Xenotransplantation, 2006, 13: 148-153

[3]

KumlienG., UllströmL., LosvallA., et al.. Clinical experience with a new apheresis filter that specifically depletes ABO blood group antibodies. Transfusion, 2006, 46: 1568-1575

[4]

TydenG., KumlienG., GenbergH., et al.. ABO incompatible kidney transplantations without splenectomy, using antigen-specific immunoadsorption and rituximab. Am J Transplant, 2005, 5: 145-148

[5]

TydénG., DonauerJ., WadströmJ., et al.. Implementation of a protocol for ABO-incompatible kidney transplantation—a three-center experience with 60 consecutive transplantations. Transplantation, 2007, 83: 1153-1155

[6]

BarenholzA., HovavA. H., FishmanY., et al.. A peptide mimetic of the mycobacterial mannosylated lipoarabinomannan: characterization and potential applications. J Med Microbiol, 2007, 56: 579-586

[7]

TangS. S., TanW. S., DeviS., et al.. Mimotopes of the Vi antigen of salmonella enterica serovar Typhi identified from phage display peptide library. Clin Diagn Lab Immunol, 2003, 10: 1078-1084

[8]

ScottJ. K., LoganathanD., EasleyR. B., et al.. A family of concanavalin A-binding peptides from a hexapeptide epitope library. Proc Natl Acad Sci USA, 1992, 89: 5398-5402

[9]

SugimuraY., HosonoM., WadaF., et al.. Screening for the preferred substrate sequence of transglutaminase using a phage-displayed peptide library: identification of peptide substrates for tgase 2 and factor xiiia. J Biol Chem, 2006, 281: 17 699-17 706

[10]

SharmaA., SahaA., BhattacharjeeS., et al.. Specific and randomly derived immunoactive peptide mimotopes of mycobacterial antigens. Clin Vaccine Immunol, 2006, 13: 1143-1154

[11]

CarterD. M., GagnonJ. N., DamlajM., et al.. Phage display reveals multiple contact sites between FhuA, an outer membrane receptor of Escherichia coli, and TonB. J Mol Biol, 2006, 357: 236-251

[12]

HouY., GuX. X.. Development of peptide mimotopes of lipooligosaccharide from nontypeable Haemophilus influenzae as vaccine candidates. J Immunol, 2003, 170: 4373-4379

[13]

LiM., YanZ., HanW., et al.. Mimotope vaccination for epitope-specific induction of anti-CD20 antibodies. Cell Immunol, 2006, 239: 136-143

[14]

ClémentM. J., FortunéA., PhaliponA., et al.. Toward a better understanding of the basis of the molecular mimicry of polysaccharide antigens by peptides: the example of shigella flexneri 5a. J Biol Chem, 2006, 281: 2317-2332

[15]

GevorkianG., SeguraE., AceroG., et al.. Peptide mimotopes of Mycobacterium tuberculosis carbohydrate immunodeterminants. Biochem J, 2005, 387: 411-417

[16]

BeninatiC., ArseniS., MancusoG., et al.. Protective immunization against group B meningococci using anti-idiotypic mimics of the capsular polysaccharide. J Immunol, 2004, 172: 2461-2468

[17]

ZhongG., SmithG. P., BerryJ., et al.. Conformational mimicry of a chlamydial neutralization epitope on filamentous phage. J Biol Chem, 1994, 269: 24183-24188

[18]

OldenburgK. R., LoganathanD., GoldsteinI. J., et al.. Peptide ligands for a sugar-binding protein isolated from a random peptide library. Proc Natl Acad Sci USA, 1992, 89: 5393-5397

[19]

WesterinkM. A., GiardinaP. C., ApicellaM. A., et al.. Peptide mimicry of the meningococcal group C capsular polysaccharide. Proc Natl Acad Sci USA, 1995, 92: 4021-4025

[20]

LangJ., ZhanJ., XuL., et al.. Identification of peptide mimetics of xenoreactive alpha-Gal antigenic epitope by phage display. Biochem Biophys Res Commun, 2006, 344: 214-220

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